Oleuropein是橄榄树中的主要酚类化合物，具有强效的抗氧化和抗炎特性。
Cas No.:32619-42-4
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Oleuropein is the major phenolic compound in the olive tree, with potent antioxidant and anti-inflammatory properties [1]. Oleuropein can cross the brain blood barrier and reduce neurotoxicity by promoting autophagy in neurons via regulation of the Ca2+-CaMKKβ-AMPK axis [2]. Oleuropein has been widely used in cell and animal models to inhibit tumor progression and inflammation[3].
In vitro, Oleuropein treatment for 72 hours significantly inhibited the proliferation of FM3 cells, HCT-116 cells and HeLa cells, with IC50 values of 274.53μM, 185.12μM and 265.28μM, respectively[4]. Treatment with 200μM Oleuropein for 72 hours significantly inhibited the viability of MCF 7 cells, reduced the expression of Bcl-2 and Bax genes, promoted the expression of p53 and induced cell apoptosis[5]. Treatment with 400μM Oleuropein for 11 days inhibited the differentiation of 3T3-L1 adipocytes, reduced the total lipid content, and suppressed the expression of PPARγ, FAS, C/EBPα and SREBP-1c genes[6].
In vivo, Oleuropein treatment via daily intraperitoneal injection at a dose of 200mg/kg for three consecutive days significantly alleviated liver necrosis and inflammatory responses induced by carbon tetrachloride, and improved the histological and plasma markers of liver damage in mice[7]. Continuous intradermal injection of 50mg/kg dose of Oleuropein for 7 consecutive days can accelerate the skin wound healing in aged male Balb/c mice[8].
References:
[1] Nediani C, Ruzzolini J, Romani A, et al. Oleuropein, a bioactive compound from Olea europaea L., as a potential preventive and therapeutic agent in non-communicable diseases[J]. Antioxidants, 2019, 8(12): 578.
[2] Gu Y, Han J. Autophagy and polyphenol intervention strategy in aging[J]. Trends in Food Science & Technology, 2023, 132: 1-10.
[3] Gorzynik-Debicka M, Przychodzen P, Cappello F, et al. Potential health benefits of olive oil and plant polyphenols[J]. International journal of molecular sciences, 2018, 19(3): 686.
[4] Samara P, Christoforidou N, Lemus C, et al. New semi-synthetic analogs of oleuropein show improved anticancer activity in vitro and in vivo[J]. European Journal of Medicinal Chemistry, 2017, 137: 11-29.
[5] Hassan Z K, Elamin M H, Omer S A, et al. Oleuropein induces apoptosis via the p53 pathway in breast cancer cells[J]. Asian Pacific Journal of Cancer Prevention, 2013, 14(11): 6739-6742.
[6] Svobodova M, Andreadou I, Skaltsounis A L, et al. Oleuropein as an inhibitor of peroxisome proliferator-activated receptor gamma[J]. Genes & nutrition, 2014, 9(1): 376.
[7] Domitrović R, Jakovac H, Marchesi V V, et al. Preventive and therapeutic effects of oleuropein against carbon tetrachloride-induced liver damage in mice[J]. Pharmacological research, 2012, 65(4): 451-464.
[8] Mehraein F, Sarbishegi M, Aslani A. Evaluation of effect of oleuropein on skin wound healing in aged male BALB/c mice[J]. Cell Journal (Yakhteh), 2014, 16(1): 25.
Oleuropein是橄榄树中的主要酚类化合物，具有强效的抗氧化和抗炎特性[1]。Oleuropein能够穿过血脑屏障，并通过调节Ca2+-CaMKKβ-AMPK轴促进神经元自噬，从而降低神经毒性[2]。Oleuropein已被广泛用于细胞和动物模型中以抑制肿瘤进展和炎症[3]。
在体外，Oleuropein处理72小时显著抑制了FM3细胞、HCT-116细胞和HeLa细胞的增殖，IC50值分别为274.53µM、185.12µM和265.28µM[4]。使用200µM的Oleuropein处理72小时，显著抑制了MCF 7细胞的活力，降低了Bcl-2和Bax基因的表达，促进了p53的表达，并诱导了细胞凋亡[5]。使用400µM的Oleuropein处理11天，抑制了3T3-L1脂肪细胞的分化，减少了总脂质含量，并降低了PPARγ、FAS、C/EBPα和SREBP-1c基因的表达[6]。
在体内，每日腹腔注射200mg/kg剂量的Oleuropein，连续三天，显著减轻了四氯化碳诱导的小鼠肝坏死和炎症反应，并改善了肝损伤的组织学及血浆标志物[7]。连续7天每日皮内注射50mg/kg剂量的Oleuropein，可加速老年雄性Balb/c小鼠的皮肤伤口愈合[8]。
| Cell experiment [1]: | |
Cell lines | HCT-116 cells |
Preparation Method | HCT-116 cells cultured in Dulbecco's Modified Eagle's Medium (DMEM), supplemented with 10% heat inactivated fetal bovine serum, 10mM Hepes, 50mM mercaptoethanol, 102U/ml penicillin/streptomycin and 5mg/ml gentamycin, at 37 °C in a humidified 5% CO2 incubator. HCT-116 cells (5×103 cells/well) were inoculated in 96-well cell culture plate and incubated at 37°C with 5% CO2 for 24h. Then, the cells were treated with different concentrations of Oleuropein (1, 10, 25, 50, 100, 200, 300, and 400μM) for 72h, respectively, and cell viability was measured. |
Reaction Conditions | 1, 10, 25, 50, 100, 200, 300, and 400μM; 72h |
Applications | Oleuropein treatment suppressed the cell viability of HCT-116 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Male BALB/cN mice |
Preparation Method | Male BALB/cN mice (3 months old; weight 23-25g) were housed in a temperature-controlled (23±2°C) room maintained on a 12/12h light/dark cycle. All mice were adaptively fed for one week. Mice were divided into 4 groups of 5 mice each. The control group (group I) received saline and group II received oleuropein 200mg/kg once daily for 3 days. Group III was administered CCl4 dissolved in corn oil (2ml/kg;10% v/v), intraperitoneally (i.p.). Oleuropein, dissolved in saline and stabilized with DMSO (1% v/v), was given i.p. at doses of 200mg/kg (groups IV) once daily for 3 consecutive days, with the last dose administered half an hour prior to CCl4 administration. Collect the liver tissues from mice for analysis. |
Dosage form | 200mg/kg/day for 3 days; i.p. |
Applications | Oleuropein treatment prevented liver damage and attenuated oxidative/nitrosative stress in mice caused by CCl4. |
References: | |
| Cas No. | 32619-42-4 | SDF | |
| 别名 | 橄榄苦苷 | ||
| 化学名 | methyl (4S,5E,6S)-4-[2-[2-(3,4-dihydroxyphenyl)ethoxy]-2-oxoethyl]-5-ethylidene-6-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4H-pyran-3-carboxylate | ||
| Canonical SMILES | CC=C1C(C(=COC1OC2C(C(C(C(O2)CO)O)O)O)C(=O)OC)CC(=O)OCCC3=CC(=C(C=C3)O)O | ||
| 分子式 | C25H32O13 | 分子量 | 540.52 |
| 溶解度 | DMF: 50 mg/ml,DMSO: 30 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 0.25 mg/ml | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.8501 mL | 9.2504 mL | 18.5007 mL |
| 5 mM | 370 μL | 1.8501 mL | 3.7001 mL |
| 10 mM | 185 μL | 925 μL | 1.8501 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet