Ginsenoside Rb1

Ginsenoside Rb1 is a natural active ingredient extracted from ginseng that can modulate the activity of multiple signaling pathways. Ginsenoside Rb1 inhibits Na⁺,K⁺-ATPase activity (IC₅₀=6.3±1.0μM). Ginsenoside Rb1 can be used in research on diseases such as cancer, cardiovascular and cerebrovascular diseases, and asthma^[1-4].

In vitro, WI-38 cells were treated with Ginsenoside Rb1 (20–30μM) and LPS (2.5–10mg/ml) for 24h. Ginsenoside Rb1 significantly reversed the LPS-induced decrease in cell viability, apoptosis, and production of pro-inflammatory factors (IL-1β, IL-6, TNF-α); Ginsenoside Rb1 downregulated miR-222 expression and inhibited NF-κB pathway activation^[5]. HUVECs were pretreated with Ginsenoside Rb1 (10–20μM) for 30min, followed by stimulation with H₂O₂ (60μM) for 1h and then cultured for another 24h. Ginsenoside Rb1 significantly reduced the number of SA-β-gal-positive cells and PAI-1 expression, while increasing eNOS expression and NO production, and restored SIRT1 expression and AMPK phosphorylation^[6].

In vivo, aged C57BL/6J mice received intraperitoneal injections of Ginsenoside Rb1 (20mg/kg; 6 days per week) for 6 weeks. Ginsenoside Rb1 significantly alleviated age-related aortic structural remodeling and DNA damage^[7]. In ovariectomized C57BL/6J mice, Ginsenoside Rb1 (12.5 or 50mg/kg; single or 7 days) was administered intraperitoneally 1 hour before ISO (200mg/kg) treatment. Ginsenoside Rb1 significantly attenuated ISO-induced myocardial injury and cardiomyocyte necrosis^[8].

References:
[1] Cao J, Zheng YQ, Liu TP, et al. Inhibitory effects of ginsenoside Rg1 and Rb1 on rat brain microsomal Na+,K(+)-ATPase activity. Zhongguo Yao Li Xue Bao. 1990 Jan;11(1):10-4.
[2] Zhang J, Cao L, Wang H, et al. Ginsenosides Regulate PXR/NF-κB Signaling and Attenuate Dextran Sulfate Sodium-Induced Colitis. Drug Metab Dispos. 2015 Aug;43(8):1181-9.
[3] Joh EH, Lee IA, Jung IH, et al. Ginsenoside Rb1 and its metabolite compound K inhibit IRAK-1 activation--the key step of inflammation. Biochem Pharmacol. 2011 Aug 1;82(3):278-86.
[4] He S, Ye H, Wang Q, et al. Ginsenoside Rb1 targets to HO-1 to improve sepsis by inhibiting ferroptosis. Free Radic Biol Med. 2025 Jan;226:13-28.
[5] Wei E, Fang X, Jia P, et al. Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 Cells. Cell Transplant. 2021 Jan-Dec;30:9636897211002787.
[6] Zheng Z, Wang M, Cheng C, et al. Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway. Mol Med Rep. 2020 Jul;22(1):247-256.
[7] Si Q, Wu L, Zhang X, et al. Ginsenoside Rb1 attenuates arterial aging by reducing DNA damage in aged mice. Exp Gerontol. 2026 Feb;214:113011.
[8] Wang Y, Tang X, Cui J, et al. Ginsenoside Rb1 mitigates acute catecholamine surge-induced myocardial injuries in part by suppressing STING-mediated macrophage activation. Biomed Pharmacother. 2024 Jun;175:116794.

Ginsenoside Rb1是一种从人参中提取的天然活性成分，可以调节多种信号通路活性。Ginsenoside可抑制Na⁺，K⁺-ATPase（IC₅₀=6.3±1.0μM）活性。Ginsenoside Rb1可用于癌症、心脑血管疾病、哮喘等疾病的研究^[1-4]。

在体外，Ginsenoside Rb1（20-30μM）与LPS（2.5-10mg/ml）处理WI-38细胞24h。Ginsenoside Rb1显著逆转了LPS引起的细胞活力降低、细胞凋亡和促炎因子（IL-1β、IL-6、TNF-α）的产生；Ginsenoside Rb1下调miR-222表达并抑制NF-κB通路激活^[5]。Ginsenoside Rb1（10-20μM）预处理HUVECs 30min，随后以H₂O₂（60μM）刺激1h并培养24h。Ginsenoside Rb1显著减少SA-β-gal阳性细胞数量和PAI-1表达，同时增加eNOS表达和NO产生、恢复SIRT1表达和AMPK磷酸化^[6]。

在体内，Ginsenoside Rb1（20mg/kg；每周6天）腹腔注射于老龄C57BL/6J小鼠，持续6周。Ginsenoside Rb1显著减轻了与年龄相关的主动脉结构重塑和DNA损伤^[7]。在ISO（200mg/kg）处理前前1小时给药，Ginsenoside Rb1（12.5或50mg/kg；单次或连续7天）腹腔注射于卵巢切除的C57BL/6J小鼠。Ginsenoside Rb1显著减轻了ISO诱导的心肌损伤和心肌细胞坏死^[8]。