Noradrenaline bitartrate monohydrate
(Synonyms: 酒石酸去甲肾上腺素; Levarterenol bitartrate monohydrate; L-Noradrenaline bitartrate monohydrate) 目录号 : GC10976
一键复制产品信息
Noradrenaline bitartrate monohydrate是一种β1选择性肾上腺素受体激动剂，EC50值为5.37μM。
Cas No.:108341-18-0
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Noradrenaline bitartrate monohydrate is a β1-selective adrenergic receptor agonist with an EC50 value of 5.37μM [1]. Noradrenaline bitartrate monohydrate enhances long-term memory consolidation in the amygdala and hippocampus through actions at α-1 and β adrenoceptors can enhance long-term memory consolidation in the amygdala and hippocampus through actions at α-1 and β adrenoceptors [2]. Noradrenaline bitartrate monohydrate has been widely used to promote the contraction of mesenteric arteries[3].
In vitro, Noradrenaline bitartrate monohydrate (1μM) treatment for 6 days protected rat dopaminergic neuronal cells from death and reduced intracellular reactive oxygen species (ROS) levels[4]. Treatment with noradrenaline bitartrate monohydrate (50μM) for 3 minutes significantly depolarized the membrane potential of pyramidal neurons in the medial prefrontal cortex[5]. 30μM of Noradrenaline bitartrate monohydrate pretreatment for 24 hours significantly attenuated H2O2-induced cell death in SH-SY5Y cells[6]. Treatment with 1μM Noradrenaline bitartrate monohydrate for 24 hours significantly reduced the release of IL-6 and TNF-alpha in microglial cells stimulated by lipopolysaccharide (LPS)[7].
References:
[1] MacGregor D A, Prielipp R C, Butterworth IV J F, et al. Relative efficacy and potency of β-adrenoceptor agonists for generating cAMP in human lymphocytes[J]. Chest, 1996, 109(1): 194-200.
[2] Ramos B P, Arnsten A F T. Adrenergic pharmacology and cognition: focus on the prefrontal cortex[J]. Pharmacology & therapeutics, 2007, 113(3): 523-536.
[3] Furness J B. The physiological relevance of constriction of mesenteric arteries by topically applied noradrenaline[J]. The Journal of physiology, 2017, 595(21): 6783.
[4] Troadec J D, Marien M, Darios F, et al. Noradrenaline provides long‐term protection to dopaminergic neurons by reducing oxidative stress[J]. Journal of neurochemistry, 2001, 79(1): 200-210.
[5] Grzelka K, Kurowski P, Gawlak M, et al. Noradrenaline modulates the membrane potential and holding current of medial prefrontal cortex pyramidal neurons via β1-adrenergic receptors and HCN channels[J]. Frontiers in cellular neuroscience, 2017, 11: 341.
[6] Yoshioka Y, Negoro R, Kadoi H, et al. Noradrenaline protects neurons against H2O2‐induced death by increasing the supply of glutathione from astrocytes via β3‐adrenoceptor stimulation[J]. Journal of Neuroscience Research, 2021, 99(2): 621-637.
[7] Färber K, Pannasch U, Kettenmann H. Dopamine and noradrenaline control distinct functions in rodent microglial cells[J]. Molecular and Cellular Neuroscience, 2005, 29(1): 128-138.
Noradrenaline bitartrate monohydrate是一种β1选择性肾上腺素受体激动剂，EC50值为5.37μM[1]。Noradrenaline bitartrate monohydrate通过作用于α-1和β肾上腺素受体，可增强杏仁核和海马的长时程记忆巩固[2]。Noradrenaline bitartrate monohydrate已被广泛用于促进肠系膜动脉的收缩[3]。
在体外，1μM的Noradrenaline bitartrate monohydrate处理6天，抑制大鼠多巴胺能神经元细胞死亡，并降低了细胞内活性氧(ROS)水平[4]。50μM的Noradrenaline bitartrate monohydrate处理3分钟，显著使内侧前额叶皮层锥体神经元的膜电位去极化[5]。30μM的Noradrenaline bitartrate monohydrate预处理SH-SY5Y细胞24小时，显著减轻了H2O2诱导的细胞死亡[6]。1μM的Noradrenaline bitartrate monohydrate处理24小时，显著降低了脂多糖(LPS)刺激的小胶质细胞中IL-6和TNF-α的释放[7]。
| Cell experiment [1]: | |
Cell lines | SH-SY5Y cells |
Preparation Method | SH-SY5Y cells were incubated with DMEM medium supplemented with 10% fetal calf serum (FCS) in a humidified atmosphere at 37°C with 5% CO2. SH-SY5Y cells were plated at a density of 0.9×104/well in 96-well tissue culture plate for 24h and were incubated for 24h in fresh culture medium containing with different concentrations of Noradrenaline bitartrate monohydrate (1, 3, 10, and 30μM). Then the culture medium was aspirated and replaced with fresh medium containing 500µM H2O2. The cell viability of SH-SY5Y cells was determined. |
Reaction Conditions | 1, 3, 10, and 30μM; 24h |
Applications | Noradrenaline bitartrate monohydrate treatment significantly reduced H2O2-induced death of SH-SY5Y cells in a dose-dependent manner. |
References: | |
| Cas No. | 108341-18-0 | SDF | |
| 别名 | 酒石酸去甲肾上腺素; Levarterenol bitartrate monohydrate; L-Noradrenaline bitartrate monohydrate | ||
| 化学名 | 4-[(1R)-2-amino-1-hydroxyethyl]benzene-1,2-diol;(2R,3R)-2,3-dihydroxybutanedioic acid;hydrate | ||
| Canonical SMILES | C1=CC(=C(C=C1C(CN)O)O)O.C(C(C(=O)O)O)(C(=O)O)O.O | ||
| 分子式 | C8H11NO3.C4H6O6.H2O | 分子量 | 337.28 |
| 溶解度 | ≥ 11.35mg/mL in DMSO | 储存条件 | 4°C, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.9649 mL | 14.8245 mL | 29.649 mL |
| 5 mM | 593 μL | 2.9649 mL | 5.9298 mL |
| 10 mM | 296.5 μL | 1.4824 mL | 2.9649 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet