Pyruvic acid (2-Oxopropanoic acid)是一种重要的有机化学中间体，也是聚合物的潜在前体。
Cas No.:127-17-3
Sample solution is provided at 25 µL, 10mM.
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Pyruvic acid (2-Oxopropanoic acid), an important organic chemical intermediate, is a potential precursor for polymers [1]. Pyruvic acid is converted into carbohydrates through gluconeogenesis, which are further transformed into fatty acids/energy, and ultimately into amino acids, such as alanine and ethanol[2]. Pyruvic acid combines the keratolytic effect of α-keto acids and the humectant effects of lactic acid, and has been widely used as a topical peeling agent to mitigate acne [3].
In vitro, Pyruvic acid treatment (1mM) for 8 hours significantly inhibited Cu2+/Zn2+-induced cell death in GT1-7 cells, alleviated mitochondrial damage, and reduced the release of cytochrome c into the cytoplasm[4]. Treatment with 10mM Pyruvic acid for 24 hours significantly increased the survival of N-2A cells under 500µM H₂O₂ conditions[5]. Pyruvic acid treatment at 5mM for 48 hours significantly reduced the tyrosinase activity in B16F10 cells, induced GSK3β phosphorylation, and activated the PI3K/AKT signaling pathway, thereby leading to a decrease in melanin synthesis[6].
In vivo, Pyruvic acid treatment via intraperitoneal injection at a single dose of 1g/kg 15 minutes before administering sodium sulfide (100mg/kg; i.p.) significantly reduced the mortality rate of mice[7].
References:
[1] Maleki N, Eiteman M A. Recent progress in the microbial production of pyruvic acid[J]. Fermentation, 2017, 3(1): 8.
[2] Pal D, Keshav A, Mazumdar B, et al. Production and recovery of pyruvic acid: recent advances[J]. Journal of The Institution of Engineers (India): Series E, 2017, 98(2): 165-175.
[3] Cotellessa C, Manunta T, Ghersetich I, et al. The use of pyruvic acid in the treatment of acne[J]. Journal of the European Academy of Dermatology and Venereology, 2004, 18(3): 275-278.
[4] Tanaka K, Shimoda M, Kawahara M. Pyruvic acid prevents Cu2+/Zn2+-induced neurotoxicity by suppressing mitochondrial injury[J]. Biochemical and biophysical research communications, 2018, 495(1): 1335-1341.
[5] Mazzio E, Soliman K F A. Pyruvic acid cytoprotection against 1-methyl-4-phenylpyridinium, 6-hydroxydopamine and hydrogen peroxide toxicities in vitro[J]. Neuroscience Letters, 2003, 337(2): 77-80.
[6] Zhou S, Sakamoto K. Pyruvic acid/ethyl pyruvate inhibits melanogenesis in B16F10 melanoma cells through PI3K/AKT, GSK3β, and ROS‐ERK signaling pathways[J]. Genes to Cells, 2019, 24(1): 60-69.
[7] Dulaney Jr M, Hume A S. Pyruvic acid protects against the lethality of sulfide[J]. Research communications in chemical pathology and pharmacology, 1988, 59(1): 133-136.
Pyruvic acid (2-Oxopropanoic acid)是一种重要的有机化学中间体，也是聚合物的潜在前体[1]。Pyruvic acid通过糖异生作用转化为碳水化合物，随后进一步转化为脂肪酸/能量，并最终转化为丙氨酸和乙醇等氨基酸[2]。Pyruvic acid结合了α-酮酸的角质剥脱作用和乳酸的保湿作用，已被广泛用作局部脱皮剂以减轻痤疮[3]。
在体外，1mM的Pyruvic acid处理GT1-7细胞8小时，显著抑制了Cu2+/Zn2+诱导的细胞死亡，减轻了线粒体损伤，并减少了细胞色素c向细胞质的释放[4]。10mM的Pyruvic acid处理N-2A细胞24小时，显著提高了细胞在500µM H2O2条件下的存活率[5]。5mM的Pyruvic acid处理B16F10细胞48小时，显著降低了酪氨酸酶活性，诱导了GSK3β磷酸化，并激活了PI3K/AKT信号通路，从而导致黑色素合成减少[6]。
在体内，在腹腔注射硫化钠100mg/kg前15分钟，单次腹腔注射1g/kg剂量的Pyruvic acid，显著降低了小鼠的死亡率[7]。
| Cell experiment [1]: | |
Cell lines | N-2A cells |
Preparation Method | N-2A cells were grown in Dulbecco's Modified Eagle Medium (DMEM) with 10% (v/v) fetal bovine serum (FBS), 4mM l-glutamine, and penicillin/streptomycin (100Units/0.1mg per ml) at 37°C in 5% CO2/atmosphere. The N-2A cells were treated with different concentrations of Pyruvic acid (0, 0.1, 1, 5, and 10mM) and H2O2 (500μM) at 37°C for 24 hours, and then the cell viability was analyzed. |
Reaction Conditions | 0, 0.1, 1, 5, and 10mM; 24h |
Applications | Pyruvic acid treatment significantly enhanced the cell viability of N-2A cells in the presence of H2O2 in a dose-dependent manner. |
References: | |
| Cas No. | 127-17-3 | SDF | |
| 别名 | 丙酮酸; Acetylformic acid | ||
| Canonical SMILES | CC(C(O)=O)=O | ||
| 分子式 | C3H4O3 | 分子量 | 88.06 |
| 溶解度 | ≥43 mg/mL in EtOH; ≥55.2 mg/mL in DMSO; ≥8.8 mg/mL in Water | 储存条件 | Store at 2-8°C,stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 11.3559 mL | 56.7795 mL | 113.5589 mL |
| 5 mM | 2.2712 mL | 11.3559 mL | 22.7118 mL |
| 10 mM | 1.1356 mL | 5.6779 mL | 11.3559 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
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