Farnesol是一种在自然界普遍存在的C15萜烯醇，是植物精油的组成部分，也是白色念珠菌的群体感应分子。
Cas No.:4602-84-0
Sample solution is provided at 25 µL, 10mM.
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Farnesol, a C15 terpene alcohol that is ubiquitous in nature, is a component of plant essential oils and is a quorum sensing molecule in Candida albicans [1]. Farnesol exerts the pro-inflammatory effect by increasing the levels of IL-6, TNF-α, and COX-2 in macrophage cells[2]. Farnesol has been widely used in various cell models and animal models to inhibit tumor growth[3].
In vitro, Farnesol treatment for 24 hours significantly inhibited the viability of A549 cells and Caco-2 cells, with IC50 values of 4.6mM and 3.9mM, respectively[4]. Treatment with 20μM Farnesol for 48 hours significantly inhibited the growth of MCF-7 cells, induced the expression of THRβ1 mRNA and protein, and prevented the binding of THRβ1 to DNA[5]. Treatment of H460 cells with 250μM Farnesol for 24 hours induced endoplasmic reticulum stress and unfolded protein response, promoted cell apoptosis, accompanied by the activation of MEK1/2[6].
In vivo, Farnesol treatment via oral administration at a dose of 100mg/kg/day for 5 weeks restored the cytokine secretion capacity of peritoneal macrophages in asthmatic mice, and reduced the levels of IL-6, TNF-α and inducible nitric oxide synthase (iNOS)[7]. Oral administration of 50mg/kg Farnesol daily for 5 weeks significantly reduced tumor growth in a prostate cancer mouse model[8].
References:
[1] Chandran S S, Kealey J T, Reeves C D. Microbial production of isoprenoids[J]. Process biochemistry, 2011, 46(9): 1703-1710.
[2] Ghosh S, Howe N, Volk K, et al. Candida albicans cell wall components and farnesol stimulate the expression of both inflammatory and regulatory cytokines in the murine RAW264. 7 macrophage cell line[J]. FEMS Immunology & Medical Microbiology, 2010, 60(1): 63-73.
[3] Jung Y Y, Hwang S T, Sethi G, et al. Potential anti-inflammatory and anti-cancer properties of farnesol[J]. Molecules, 2018, 23(11): 2827.
[4] Yilmaz Öztürk B, Feyzullazade N, Dağ İ, et al. The investigation of in vitro effects of farnesol at different cancer cell lines[J]. Microscopy Research and Technique, 2022, 85(8): 2760-2775.
[5] Duncan R E, Archer M C. Farnesol induces thyroid hormone receptor (THR) β1 but inhibits THR-mediated signaling in MCF-7 human breast cancer cells[J]. Biochemical and biophysical research communications, 2006, 343(1): 239-243.
[6] Joo J H, Liao G, Collins J B, et al. Farnesol-induced apoptosis in human lung carcinoma cells is coupled to the endoplasmic reticulum stress response[J]. Cancer research, 2007, 67(16): 7929-7936.
[7] Ku C M, Lin J Y. Farnesol, a sesquiterpene alcohol in herbal plants, exerts anti‐inflammatory and antiallergic effects on ovalbumin‐sensitized and‐challenged asthmatic mice[J]. Evidence‐Based Complementary and Alternative Medicine, 2015, 2015(1): 387357.
[8] Park J S, Kwon J K, Kim H R, et al. Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways[J]. International journal of molecular medicine, 2014, 33(5): 1169-1176.
Farnesol是一种在自然界普遍存在的C15萜烯醇，是植物精油的组成部分，也是白色念珠菌的群体感应分子[1]。Farnesol通过增加巨噬细胞中IL-6、TNF-α和COX-2的水平发挥促炎作用[2]。Farnesol已被广泛用于各种细胞模型和动物模型，以抑制肿瘤生长[3]。
在体外，Farnesol处理24小时显著抑制了A549细胞和Caco-2细胞的活力，IC50值分别为4.6mM和3.9mM[4]。使用20µM的Farnesol处理48小时，显著抑制了MCF-7细胞的生长，诱导了THRβ1 mRNA和蛋白的表达，并阻止了THRβ1与DNA的结合[5]。使用250µM的Farnesol处理H460细胞24小时，诱导了内质网应激和未折叠蛋白反应，促进了细胞凋亡，并伴有MEK1/2的激活[6]。
在体内，每日口服给予100mg/kg剂量的Farnesol，持续5周，恢复了哮喘小鼠腹腔巨噬细胞的细胞因子分泌能力，并降低了IL-6、TNF-α和诱导型一氧化氮合酶（iNOS）的水平[7]。每日口服50mg/kg剂量的Farnesol，持续5周，显著降低了前列腺癌小鼠模型中的肿瘤生长[8]。
| Cell experiment [1]: | |
Cell lines | Caco-2 cells |
Preparation Method | The growth and maintenance of Caco-2 cells were carried out in a modified Dubecco Eagle medium (DMEM) containing 10% FBS and 1% antibiotics, and the cells were cultured at 37°C, 5% CO2 and humidity conditions. Adjust the cell concentration to 5×104 cells/ml, and inoculate it in 96-well plates at a volume of 100μl/well. Treat the cells with different concentrations of Farnesol (0.01, 0.025, 0.05, 0.1, 0.5, 1, 2, 5, and 10mM) for 24 hours, and then analyze the cell viability. |
Reaction Conditions | 0.01, 0.025, 0.05, 0.1, 0.5, 1, 2, 5, and 10mM; 24h |
Applications | Farnesol treatment inhibited the cell viability of Caco-2 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Male nude mice |
Preparation Method | Five-week-old male nude mice weighing 20-30 grams were raised in a standard sterile environment with a temperature of 23±5℃ and a relative humidity of 40±10%. A density of 5×106 cells/ml of DU145 cells were injected subcutaneously into the lateral abdomen of the nude mice. All mice received oral treatment starting from the day of tumor implantation and continued for 5 weeks. The control group mice were orally administered 0.2ml of corn oil daily for 5 weeks. The treatment group mice were orally administered 50mg/kg of Farnesol daily for 5 weeks, and the tumor growth was analyzed. |
Dosage form | 50mg/kg/day for 5 weeks; p.o. |
Applications | Farnesol treatment significantly decreased the tumor volume in the DU145 cell- xenograft mouse models. |
References: | |
| Cas No. | 4602-84-0 | SDF | |
| 别名 | 法呢醇 | ||
| Canonical SMILES | C/C(C)=C\CC/C(C)=C/CC/C(C)=C/CO | ||
| 分子式 | C15H26O | 分子量 | 222.37 |
| 溶解度 | DMF: 20 mg/ml,DMSO: 10 mg/ml,Ethanol: 30 mg/ml,Ethanol:PBS (pH 7.2) (1:3): 0.25 mg/ml | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.497 mL | 22.485 mL | 44.9701 mL |
| 5 mM | 899.4 μL | 4.497 mL | 8.994 mL |
| 10 mM | 449.7 μL | 2.2485 mL | 4.497 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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