Polyethylenimine (branched) (MW 10000) (PEI (branched) (MW 10000)) 是一种阳离子聚合物,广泛用于非病毒核苷酸递送试剂。
Cas No.:9002-98-6
Sample solution is provided at 25 µL, 10mM.
Polyethylenimine (branched) (MW 10000) (PEI (branched) (MW 10000)) is a cationic polymer widely used as a nonviral nucleotide delivery reagent[1].
Branched polyethylenimine (MW 10000) exhibits lower cytotoxicity compared to high molecular weight PEI, but its transfection efficiency is limited [1].
Branched polyethylenimine (MW 10000) is often cross-linked with biodegradable linkers to maintain good nucleic acid condensation while improving biocompatibility [1].
References:
[1]. Casper J, et al. Polyethylenimine (PEI) in gene therapy: Current status and clinical applications. J Control Release. 2023 Oct;362:667-691.
| Cas No. | 9002-98-6 | SDF | |
| 别名 | PEI (branched) (MW 10000) | ||
| 分子式 | (C2H5N)n | 分子量 | 10000 (Average) |
| 溶解度 | DMSO : 200 mg/mL (Need ultrasonic); H2O : 142.86 mg/mL (Need ultrasonic) | 储存条件 | RT |
| Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 100 μL | 500 μL | 1 mL |
| 5 mM | 20 μL | 100 μL | 200 μL |
| 10 mM | 10 μL | 50 μL | 100 μL |
| 第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
| 给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
| 第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: An oil
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
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