LysoSensor™ Yellow/Blue DND-160 (PDMPO)

The LysoSensor® series of probes are fluorescent probes used to detect the pH value of acidic compartments in living cells (such as lysosomes and acrosomes). These probes exhibit acidophilic staining and accumulate in acidic organelles through protonation. This acidophilicity of the probes overcomes the fluorescence quenching effect of the dye, resulting in stronger fluorescence emission. Therefore, compared to the LysoTracker series of probes, the fluorescence intensity of the LysoSensor® series of probes increases in a pH-dependent manner (towards acidity) with the degree of organelle acidification.

This product, LysoSensor™ Yellow/Blue DND-160 (PDMPO), is a ratiometric probe for measuring lysosomal pH. It is a pH-dependent fluorescent probe in the LysoTracker® series, displaying dual excitation and dual emission wavelengths. This probe produces blue fluorescence in a neutral environment (Ex/Em = 329 nm/440 nm), but emits yellow fluorescence (Ex/Em = 384 nm/540 nm) when the environment becomes more acidic. This product is supplied as a 1 mM stock solution dissolved in anhydrous DMSO.

LysoSensor^®系列探针是对活细胞中的酸性区室（如溶酶体、顶体精子）pH值进行检测的一类荧光探针，该类探针的染色具有向酸性，可通过质子化作用在酸性细胞器内累积。该类探针的这种向酸性可解除染料的荧光淬灭性，使其所发射的荧光强度更强。因此，与LysoTracker系列探针相比，LysoSensor^®系列探针随着细胞器的酸化程度其荧光强度呈pH依赖型(向酸)增强。

本品LysoSensor™ Yellow/Blue DND-160 (PDMPO)是一种用于测定溶酶体pH值的比率测量型探针，也是LysoTracker^®系列探针中的一种pH依赖型显示双激发和双发射波长的荧光探针，该探针在中性环境中产生蓝色荧光（Ex/Em=329 nm/440 nm），但当所处环境变为偏酸性时，则发射黄色荧光（Ex/Em=384 nm/540 nm）。本品以溶于无水DMSO的1 mM储存液形式提供。