Heparinase II

Heparinase II is a glycosidase targeting heparin and heparan sulfate. Heparinase II is promising for research of glycosaminoglycan structure-function analysis, anticoagulant agents and tumor microenvironment modulation^[1].

Product Information

Calcium ions inhibit heparinase II activity. Heparinase II cleaves sulfated polysaccharide chains containing 1-4 linkages at the junctions of hexosamine and uronic acid residues (including iduronic acid and glucuronic acid residues) via an elimination mechanism. This reaction produces oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids, detectable by UV spectroscopy at 232 nm. The enzyme also cleaves the antithrombin III-binding pentasaccharide domain in the heparin molecule.

Dissolution
Use 1 mg/mL dissolved in water; fresh preparation is recommended. Alternatively, dissolve according to the specific experimental guidelines.

Reaction Conditions

1. Stable pH range: 5.0-9.0, optimal pH: 7.0-7.5

2. Stable temperature range: 20-37℃, optimal temperature: 30℃

Note: Incubation time and enzyme concentration need to be determined based on practical experience when digesting the substrate.

References:
[1]. Shriver Z, et al. Heparinase II from Flavobacterium heparinum. Role of cysteine in enzymatic activity as probed by chemical modification and site- directed mutagenesis. J Biol Chem. 1998 Sep 4;273(36):22904-12.

产品信息
钙离子会抑制肝素酶 II 活性。肝素酶 II 通过消除机制切割硫酸化多糖链，该多糖链含有 1-4 个连接，连接处为己糖胺和糖醛酸残基（包括艾杜糖醛酸和葡萄糖醛酸残基）。该反应生成含有不饱和糖醛酸的寡糖产物（主要是二糖），可通过 232 nm 的紫外光谱检测。该酶还可切割肝素分子中的抗凝血酶 III 结合五糖结构域。

溶解
可使用 1 mg/mL 溶于水，建议新鲜配制。或根据具体实验参考文献溶解。

反应条件
1. pH 稳定范围 5.0-9.0 ，最适 pH 7.0-7.5
2. 温度稳定范围 20-37℃，最适温度 30℃
注：酶切底物时需要根据实际经验确定孵育时间及酶浓度。