Freund’s Adjuvant
(Synonyms: 弗氏完全佐剂) 目录号 : GF11329Complete Freund's adjuvant (CFA) 是因发现者 Jules T. Freund 而获名的、与抗原乳化的免疫佐剂，用于增强动物对抗原 (antigen) 的免疫反应。Complete Freund's adjuvant (CFA) 也是 Th1 型免疫反应的诱导剂和 TLR 配体。Complete Freund's adjuvant (CFA) 包含热杀死的非活性化结核杆菌，并由石蜡油包水乳剂组成。Complete Freund's adjuvant (CFA) 刺激强烈且持久的免疫反应，可用于诱导小鼠的持续性炎症疼痛模型、实验性自身免疫性心肌炎 (EAM) 模型等。Incomplete Freund's adjuvant (IFA)是 Freund's Adjuvant 的另一种类型，刺激的免疫反应较弱。
Cas No.:9007-81-2
Sample solution is provided at 25 µL, 10mM.
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Complete Freund's adjuvant (CFA) is an immunoadjuvant emulsified with antigen by its discoverer Jules T. Freund to enhance an animal's immune response to an antigen. Complete Freund's adjuvant (CFA) is also an inducer of the Th1 immune response and a ligand of TLRs. Complete Freund's adjuvant (CFA) contains heat-killed inactive tuberculosis bacilli and consists of a paraffin oil-in-water emulsion. Complete Freund's adjuvant (CFA) stimulates a strong and durable immune response and can be used to induce persistent inflammatory pain models in mice, experimental autoimmune myocarditis (EAM) models, and more. Incomplete Freund's adjuvant (IFA) is another type of Freund's Adjuvant that stimulates a weaker immune response.
Complete Freund's adjuvant (CFA) contains numerous microbial TLR ligands (such as TLRs 2, 4, and 9), is a potent inducer of a Th1-type immune response[1].
Complete Freund's adjuvant (CFA) contains numerous microbial TLR ligands (such as TLRs 2, 4, and 9), is a potent inducer of a Th1-type immune response[1].
Complete Freund's adjuvant (CFA) can elicit a robust antibody response to T-cell-dependent antigens in TLR-deficient mice, which is an T-cell-dependent, antigen-specific antibody response[1].
Complete Freund's adjuvant (CFA) emulsified with Myosin or Myocarditogenic peptide, then subcutaneously injected (two injections with 7 days apart) into A/J, BALB/c, and IL6KO BALB/c male mice to induce experimental autoimmune myocarditis (EAM) mouse model[1].
Complete Freund's adjuvant (CFA) widely involves in modeling immunization-induced animal models such as collagen-induced arthritis (CIA), experimental autoimmune encephalomyelitis (EAE), experimental autoimmune thyroiditis (EAT), experimental autoimmune uveitis (EAU), and EAM[1].
Complete Freund's adjuvant (CFA) plus Thyroglobulin to induce thyroiditis, whereas Incomplete Freund's adjuvant (IFA) serves as a sufficient adjuvant for thyroid lesions[1].
Complete Freund's adjuvant (CFA) is injected into the plantar area of the hind paw of C57BL/6J mice to induce inflammatory pain mouse model[2].
Complete Freund's adjuvant (CFA) injection on the side contralateral results optogenetic inhibition of ventral pallidum (VP) cholinergic (ChAT) system neurons, and significantly reduces the number of c-Fos-positive neurons in the VP on the photo-inhibited side[2].
Complete Freund's adjuvant (CFA) has low plasma clearance (22%) in normal rats[4]. Complete Freund's adjuvant (CFA) has low plasma clearance (22%)[4].
Administration: 5mg/ml • s.c. • twice
(2) On days 0 and 7, mice received subcutaneous injections peptide emulsified in CFA supplemented with 5 mg/mL of Mycobacterium tuberculosis, strain H37Ra.
Molecular changes: Test serum levels of total IgG, IgG1, IgG2a, IgG2b, IL-1b and IL6.
Administration: 20 μl • s.c. • once
(2) Tests PWT and PWL for both hind paws corresponding to before and 24, 48, 72 h after CFA injection.
GLPBIO has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | A/J, BALB/c, and IL6KO BALB/c male mice[1] |
| Dosage: | |
| Administration: | Subcutaneous Injectionon (sc); injected at days 0 and 7. EAM severity was analyzed by histology on day 21 (Repeated administration of CFA to animals can cause multiple injection-site effects, the most often observed are on site granulomas and pain) |
| Result: | Increased the relative proportion of CD11b+F4/80−monocytes and CD11b+Gr1+ granulocytes. Expanded monocytes in the heart and in the spleen and those monocytes in the spleen make less IL-10 as well as more IL-6. |
[1]. Fontes JA, et al. Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL-6 production during initiation of the immune response. Immun Inflamm Dis. 2017 Jun;5(2):163-176.
[2]. Ji YW, et al. Plasticity in ventral pallidal cholinergic neuron-derived circuits contributes to comorbid chronic pain-like and depression-like behaviour in male mice. Nat Commun. 2023 Apr 17;14(1):2182.
[3]. Sun T, et al. Gastrodin relieved complete Freund's adjuvant-induced spontaneous pain by inhibiting inflammatory response. Int Immunopharmacol. 2016 Dec;41:66-73.
[4]. Projean D, et al. Use of Freund's Complete Adjuvant (FCA) in inflammatory pain models: consequences on the metabolism and pharmacokinetics of the non-peptidic delta receptor agonist SNC80 in the rat. Xenobiotica. 2007 Aug;37(8):870-83.
| Cas No. | 9007-81-2 | SDF | |
| 别名 | 弗氏完全佐剂 | ||
| 分子式 | 分子量 | ||
| 溶解度 | 储存条件 | 2-8°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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