Esterase

Esterase, from pig liver, namely carboxylesterase, is responsible for the hydrolysis of carboxylesters into the corresponding alcohol and carboxylic acid. Esterase is principally expressed in tissues that maintain a barrier function, including the epithelia of the lung and gut, liver, kidney and skin^[1]. Esterase is commonly used in vitro to verify whether esterases can hydrolyze or activate substrates, probes, or prodrugs. Esterase from porcine liver (0-25pg; 0-625amol) is used as a standard enzyme, with Fluorescein diacetate (FDA) as the fluorescent substrate, to establish a standard curve for esterase activity and to detect esterase activity in BY-2 tobacco cells and spruce embryogenic cells^[2]. Esterase (10µL; 1.66U/µL) is used to pretreat the synthesized probe for 3.5h to induce de-esterification in advance and evaluate changes in background fluorescence^[3].

References:
[1] Hatfield MJ, Umans RA, Hyatt JL, et al. Carboxylesterases: General detoxifying enzymes. Chem Biol Interact. 2016;259(Pt B):327-331.
[2] Vitecek J, Petrlova J, Adam V, Havel L, Kramer KJ, Babula P, Kizek R. A Fluorimetric Sensor for Detection of One Living Cell. Sensors. 2007; 7(3):222-238.
[3] Watkins RW, Lavis LD, Kung VM, Los GV, Raines RT. Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells. Org Biomol Chem. 2009;7(19):3969-3975.

来自猪肝的Esterase，即羧酸酯酶，负责将羧酸酯水解成相应的醇和羧酸。Esterase主要在维持屏障功能的组织中表达，包括肺、肠、肝、肾和皮肤的上皮细胞。Esterase常用于体外验证酯酶是否可水解/激活底物、探针或前药。来自猪肝的Esterase（0-25pg；0-625amol）作为标准酶，以二乙酸荧光素（FDA）为荧光底物，用于建立酯酶活性的标准曲线，并检测 BY-2 烟草细胞和云杉胚性细胞中的酯酶活性^[2]。Esterase (10µL; 1.66U/µL)预处理3.5h，提前去酯化合成探针，以评估背景荧光的变化^[3]。