CY5-Sodium taurocholate

CY5-Sodium taurocholate is a fluorescent probe formed by covalently labeling the infrared fluorescent dye Cyanine 5 (CY5) onto sodium taurocholate molecules. Sodium taurocholate is an important component of bile acid salts, participating in lipid emulsification, cholesterol metabolism, and bile circulation. CY5 labeling allows for the direct tracking of bile acid absorption, distribution, and metabolism in in vivo and in vitro systems, providing a tool for studying the physiological functions and disease mechanisms of bile acids.

Physicochemical Properties and Structural Characteristics: CY5-Sodium taurocholate exhibits good water solubility and is stable in physiological buffers and cell culture media. The CY5 excitation peak is approximately 650 nm, and the emission peak is approximately 670 nm. It has high fluorescence intensity and excellent photostability, making it suitable for in vivo and in vitro imaging experiments. The labeling forms a covalent bond between the CV5 active group and the hydroxyl or amino groups of the taurocholate molecule, while maintaining the amphoteric molecular properties and physiological activity of the bile acid molecule. Its small molecular size facilitates the study of bile acid migration and absorption in cell membranes and blood.

Application areas: Bile acid metabolism research: tracking the distribution of taurocholic acid in the body, liver, and intestines; Lipid emulsification and absorption analysis: studying the role of bile acids in lipid emulsification and lipid absorption; Disease model research: used for in vivo tracing in models of liver diseases, gallstones, and lipid metabolism disorders; Nanocarriers and drug tracing: used for in vivo distribution and targeting analysis of bile acid-modified carriers or drugs; Fluorescence imaging experiments: combining microscopy and near-infrared imaging technology to achieve visualization of bile acids in tissues and cells.

CY5-Sodium taurocholate 是将红外荧光染料 Cyanine 5(CY5)共价标记于牛磺胆酸钠(Sodiumtaurocholate)分子形成的荧光探针。牛磺胆酸钠是胆汁酸盐的重要组成部分，参与脂质乳化、胆固醇代谢及胆汁循环。通过CY5标记，可在体内和体外系统中直观追踪胆汁酸的吸收、分布及代谢，为胆汁酸生理功能和疾病机制研究提供工具。
理化性质与结构特性:
CY5-Sodium taurocholate 水溶性良好，可在生理缓冲液及细胞培养液中稳定存在。CY5激发峰约650nm，发射峰约670nm，荧光亮度高，光稳定性优良，适合体内外成像实验。标记通过CV5活性基团与牛磺胆酸分子羟基或氨基形成共价键，同时保持胆酸分子的两性分子特性及生理活性，分子小巧便于胆汁酸在细胞膜和血液中的迁移和吸收研究。
应用领域:
胆汁酸代谢研究:追踪牛磺胆酸在体内循环、肝脏及肠道的分布;脂质乳化与吸收分析:研究胆汁酸在脂质乳化和脂质吸收中的作用;疾病模型研究:用于肝脏疾病、胆结石及脂代谢紊乱模型中的体内示踪;纳米载体与药物示踪:用于胆汁酸修饰载体或药物在体内分布和靶向性分析;荧光成像实验:结合显微镜、近红外成像技术，实现胆汁酸在组织和细胞中的可视化研究。