4-ethylphenyl sulfate是一种口服有效且能穿透血脑屏障的肠道微生物代谢物。
Cas No.:85734-98-1
Sample solution is provided at 25 µL, 10mM.
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4-ethylphenyl sulfate is an orally active and blood-brain barrier-penetrating intestinal microbial metabolite [1]. N-4-Ethylphenyl sulfate acts as a protein-bound uremic toxin that causes endothelial dysfunction and leads to chronic kidney disease [2]. 4-Ethylphenyl sulfate is widely used as a diagnostic biomarker for intestinal flora imbalance and autism, and is used as a reference substance to develop liquid chromatography/electrospray ionization methods for the identification of related compounds[3].
In vitro, 4-ethylphenyl sulfate treatment (10mM) for 72 hours significantly reduced the viability of HCT-116 cells, accompanied by cell volume reduction, formation of intracellular vesicles, and damage to cell membrane integrity[4].
References:
[1] Needham B D, Funabashi M, Adame M D, et al. A gut-derived metabolite alters brain activity and anxiety behaviour in mice[J]. Nature, 2022, 602(7898): 647-653.
[2] Verma D, Hashmi S Z H, Lakshmi G, et al. Electrochemical sensor based on polydopamine-molecularly imprinted polymer for detection of 4-ethylphenyl sulfate “a novel gut metabolite”: Fabrication, characterization, and performance evaluation in human urine[J]. Microchemical Journal, 2023, 193: 108964.
[3] Sandoni L, Asta L, Giompaolo N, et al. The Role of Microbiota Metabolites Propionic Acid, p‐Cresol, and 4‐Ethylphenyl Sulfate in Autism Susceptibility: A Systematic Review[J]. Autism Research, 2026: e70237.
[4] Jaiswal J, Srivastav A K, Kushwaha M, et al. Gut Microbial Metabolite 4-Ethylphenylsulfate Is Selectively Deleterious and Anticancer to Colon Cancer Cells[J]. Journal of Medicinal Chemistry, 2025, 68(10): 10425-10438.
4-ethylphenyl sulfate是一种口服有效且能穿透血脑屏障的肠道微生物代谢物[1]。4-ethylphenyl sulfate作为一种蛋白结合性尿毒症毒素，可导致内皮功能障碍并引发慢性肾脏病[2]。4-ethylphenyl sulfate被广泛用作肠道菌群失调和自闭症的诊断生物标志物，并作为参考物质用于开发液相色谱/电喷雾电离方法以鉴定相关化合物[3]。
在体外，10mM的4-ethylphenyl sulfate处理HCT-116细胞72小时，显著降低了细胞活力，并伴随细胞体积缩小、细胞内囊泡形成以及细胞膜完整性受损[4]。
| Cell experiment [1]: | |
Cell lines | HCT-116 cells |
Preparation Method | HCT-116 cells were cultured at 37°C and 5% CO2 in McCoy 5A medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin. Cells were plated at a density of 2.5×104 cells/ml in a 96-well plate with growth medium for 24h, and then were incubated with the different concentrations of 4-ethylphenyl sulfate (0, 1, 2.5, 5, and 10mM) for 24, 48, and 72h, respectively, the cell viability was analyzed. |
Reaction Conditions | 0, 1, 2.5, 5, and 10mM; 24, 48, and 72h |
Applications | 4-ethylphenyl sulfate treatment significantly inhibited the cell viability of HCT-116 cells in a concentration- and time-dependent manner. |
References: | |
| Cas No. | 85734-98-1 | SDF | |
| 化学名 | 4-ethylphenyl hydrogen sulfate | ||
| Canonical SMILES | OS(OC1=CC=C(CC)C=C1)(=O)=O | ||
| 分子式 | C8H10O4S | 分子量 | 202.23 |
| 溶解度 | ≥ 20.2mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.9449 mL | 24.7243 mL | 49.4486 mL |
| 5 mM | 989 μL | 4.9449 mL | 9.8897 mL |
| 10 mM | 494.5 μL | 2.4724 mL | 4.9449 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >97.00% Appearance: A solid
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