Soyasapogenol B是一种具有多种生物学活性的大豆成分。
Cas No.:595-15-3
Sample solution is provided at 25 µL, 10mM.
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Soyasapogenol B is a soy-derived component with multiple biological activities. Soyasapogenol B can modulate cellular autophagy, apoptosis, inflammation, and oxidative damage[1-2]. Soyasapogenol B is applicable for research related to colorectal cancer, clear cell renal cell carcinoma, and neurodegenerative diseases[3-4].
In vitro, Soyasapogenol B (0.5-3mM) incubation with Caco-2 cells for 4 hours. Soyasapogenol B significantly inhibited epithelial sodium channel (ENaC)-mediated sodium absorption. The uptake of Soyasapogenol B in Caco-2 cells was concentration-dependent, and Soyasapogenol B inhibited cell viability[5]. Treatment of differentiated 3T3-L1 adipocytes with Soyasapogenol B (6.25-12.5μM) for 10 days. Soyasapogenol B significantly reduced intracellular triglyceride content while increasing the levels of glycerol and free fatty acids in the culture medium[6].
In vivo, dietary administration of Soyasapogenol B (0.02%; mixed in feed) to 12-week-old male C57BL/6 mice for 8 weeks significantly increased the animals' grip strength, exercise endurance, and the weights of the quadriceps and gastrocnemius muscles[7]. In 6-week-old male ICR mice, Soyasapogenol B (10mg/kg) was orally administered once daily for 3 days, starting 24 hours after the seventh intraperitoneal injection of LPS (8μg/kg). Soyasapogenol B significantly restored the LPS-induced impairments in spontaneous alternation behavior and latency time, increased the expression of brain-derived neurotrophic factor (BDNF) and CREB phosphorylation in the hippocampus, and inhibited NF-κB activation and TNF-α expression[8].
References:
[1] Sasaki K, Minowa N, Kuzuhara H, et al. Preventive effects of soyasapogenol B derivatives on liver injury in a concanavalin A-induced hepatitis model. Bioorg Med Chem. 2005 Aug 15;13(16):4900-11.
[2] Yun HM, Lee JY, Kim SH, et al. Effects of Triterpene Soyasapogenol B from Arachis hypogaea (Peanut) on Differentiation, Mineralization, Autophagy, and Necroptosis in Pre-Osteoblasts. Int J Mol Sci. 2022 Jul 27;23(15):8297.
[3] Zhang W, Popovich DG. Effect of soyasapogenol A and soyasapogenol B concentrated extracts on HEP-G2 cell proliferation and apoptosis. J Agric Food Chem. 2008 Apr 23;56(8):2603-8.
[4] Evidente A, Cimmino A, Fernández-Aparicio M, et al. Soyasapogenol B and trans-22-dehydrocam- pesterol from common vetch (Vicia sativa L.) root exudates stimulate broomrape seed germination. Pest Manag Sci. 2011 Aug;67(8):1015-22.
[5] Hu J, Reddy MB, Hendrich S, et al. Soyasaponin I and sapongenol B have limited absorption by Caco-2 intestinal cells and limited bioavailability in women. J Nutr. 2004 Aug;134(8):1867-73.
[6] Iwamoto K, Kamo S, Takada Y, et al. Soyasapogenols reduce cellular triglyceride levels in 3T3-L1 mouse adipocyte cells by accelerating triglyceride lipolysis. Biochem Biophys Rep. 2018 Sep 27;16:44-49.
[7] Ahn BM, Noh Y, Lee JJ, et al. Soyasapogenol B prevents sarcopenia by increasing skeletal muscle mass and function through the Sirt1/PGC-1α and PI3K pathway. Biomed Pharmacother. 2025 Aug;189:118316.
[8] Lee HJ, Lim SM, Ko DB, et al. Soyasapogenol B and Genistein Attenuate Lipopolysaccharide-Induced Memory Impairment in Mice by the Modulation of NF-κB-Mediated BDNF Expression. J Agric Food Chem. 2017 Aug 16;65(32):6877-6885.
Soyasapogenol B是一种具有多种生物学活性的大豆成分。Soyasapogenol B可调节细胞自噬、凋亡、炎症和氧化损伤[1-2]。Soyasapogenol B可用于结直肠癌、透明细胞肾细胞癌和神经退行性疾病的相关研究[3-4]。
在体外，Soyasapogenol B（0.5-3mM）孵育Caco-2细胞4小时，可显著抑制上皮钠通道（ENaC）介导的钠离子吸收。Soyasapogenol B在Caco-2细胞中的摄取呈浓度依赖性，且可抑制细胞活力[5]。Soyasapogenol B（6.25-12.5μM）处理分化后的3T3-L1脂肪细胞10天，可显著降低细胞内的甘油三酯含量，同时提高培养基中的甘油和游离脂肪酸水平[6]。
在体内，Soyasapogenol B（0.02%；掺入饲料）饲喂处理12周龄起始的C57BL/6雄性小鼠8周，显著增加了小鼠的握力、运动耐力以及股四头肌和腓肠肌的重量[7]。Soyasapogenol B（10mg/kg）在第7次腹腔注射LPS（8μg/kg）后24小时开始，每天一次口服给药，持续3天，用于处理6周龄的ICR雄性小鼠。Soyasapogenol B显著恢复了LPS造成的小鼠自发交替行为和潜伏时间，增加了海马体中脑源性神经营养因子（BDNF）的表达和CREB磷酸化，并抑制了NF-κB的激活和TNF-α的表达[8]。
| Cell experiment [1]: | |
Cell lines | 3T3-L1 mouse preadipocyte cells |
Preparation Method | 3T3-L1 cells were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37°C under 5% CO₂. Differentiation into adipocytes was induced using a reagent containing insulin, dexamethasone, and 3-isobutyl-1-methylxanthine. Differentiated adipocytes were then treated with Soyasapogenol B (6.25-12.5µM). |
Reaction Conditions | 6.25-12.5µM; 10 days. |
Applications | Soyasapogenol B significantly reduced the cellular triglyceride content. Soyasapogenol B increased the levels of glycerol and free fatty acids in the culture medium. Soyasapogenol B did not alter the protein expression level of adipose triglyceride lipase (ATGL). Soyasapogenol B increased the secretion of adiponectin and significantly decreased the secretion of resistin. |
| Animal experiment [2]: | |
Animal models | ICR mice |
Preparation Method | Mice were intraperitoneally injected with LPS (8μg/kg) once a day for 7 times. Oral administration of Soyasapogenol B (10mg/kg) began 24 hours after the seventh LPS injection and was continued once daily for 3 days. Memory behavior was measured 18 hours after the final administration of the test agent. |
Dosage form | 10mg/kg; p.o.; once daily for 3 days. |
Applications | Soyasapogenol B significantly restored LPS-impaired spontaneous alternation behavior and latency time. Soyasapogenol B increased brain-derived neurotrophic factor (BDNF) expression and CREB phosphorylation in the hippocampus. Soyasapogenol B inhibited NF-κB activation and TNF-α expression in the hippocampus. |
References: | |
| Cas No. | 595-15-3 | SDF | |
| 别名 | 大豆甾醇B | ||
| Canonical SMILES | C[C@]12[C@]3(C([C@@]4([H])[C@](C)([C@@H](CC(C)(C)C4)O)CC3)=CC[C@]1([H])[C@@]5([C@@]([C@](C)([C@@H](O)CC5)CO)([H])CC2)C)C | ||
| 分子式 | C30H50O3 | 分子量 | 458.72 |
| 溶解度 | DMSO : 50 mg/mL (109.00 mM; Need ultrasonic) | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.18 mL | 10.8999 mL | 21.7998 mL |
| 5 mM | 436 μL | 2.18 mL | 4.36 mL |
| 10 mM | 218 μL | 1.09 mL | 2.18 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
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计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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