Diphyllin is a lignan that has been found in C. collinus and has diverse biological activities.1,2,3 It reduces viral titers in Vero cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; EC50 = 1.92 ?M).1 It inhibits bovine chromaffin granule V-ATPase (IC50 = 17 nM) and lysosomal acidification in primary human osteoclasts.2 Diphyllin (1, 5, and 10 ?M) induces apoptosis in and inhibits the growth of SGC-7901 gastric adenocarcinoma cells.3 In vivo, diphyllin (20 mg/kg) reduces intratumoral expression of V-ATPase and tumor volume in an SGC-7901 mouse xenograft model.
1.Stefanik, M., Strakova, P., Haviernik, J., et al.Antiviral activity of vacuolar ATPase blocker diphyllin against SARS-CoV-2Microorganisms9(3)471(2021) 2.Sorensen, M.G., Henriksen, K., Neutzsky-Wulff, A.V., et al.Diphyllin, a novel and naturally potent V-ATPase inhibitor, abrogates acidification of the osteoclastic resorption lacunae and bone resorptionJ. Bone Miner. Res.22(10)1640-1648(2007) 3.Shen, W., Zou, X., Chen, M., et al.Effects of diphyllin as a novel V-ATPase inhibitor on gastric adenocarcinomaEur. J. Pharmacol.667(1-3)330-338(2011)