HBC620 is an HBC-like fluorophore, a GFP fluorophore-like synthetic dye, with a maximum excitation wavelength of 577nm and a maximum emission wavelength of 620nm [1]. HBC620 can be used as an RNA tag, which is bright, stable, and colorful, and capable of imaging a variety of RNAs in living cells[2]. The unique fluorescence imaging channel provided by HBC620 has been widely used in the engineering of luminometric sensor platforms for multiple miRNA imaging[3]. HBC620 can be used for in situ localization of tumor-specific protease and animal optical in vivo imaging analysis experiments[4].
References:
[1] Chen X, Zhang D, Su N, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs[J]. Nature Biotechnology, 2019, 37(11): 1287-1293.
[2] Tang H, Peng J, Peng S, et al. Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers[J]. Chemical Science, 2022, 13(47): 14032-14040.
[3] Yin P, Ge M, Xie S, et al. A universal orthogonal imaging platform for living-cell RNA detection using fluorogenic RNA aptamers[J]. Chemical Science, 2023, 14(48): 14131-14139.
[4] Xie X, Sun Y, Peng J, et al. Collagen Anchoring Protein-Nucleic Acid Chimeric Probe for In Situ In Vivo Mapping of a Tumor-Specific Protease[J]. Analytical Chemistry, 2023, 95(50): 18487-18496.
HBC620是一种类HBC荧光团，属于合成型GFP荧光团类似物，最大激发波长为577nm，最大发射波长为620nm[1]。HBC620可作为RNA标记物，具有亮度高、稳定性强和色彩鲜艳的特点，能够实现对活细胞中多种RNA的成像[2]。HBC620提供的独特荧光成像通道已广泛应用于多 miRNA 成像的发光传感器平台构建[3]。HBC620可用于肿瘤特异性蛋白酶的原位定位及动物光学活体成像分析实验[4]。