5-BrdU (5-bromo-2-deoxundine5-BrdU) is a synthetic thymidine bromide analog that is commonly used to measure DNA synthesis and label dividing cells [1]. 5-BrdU can selectively insert into cellular DNA in place of thymidine during the S phase and can be used to study signaling pathways and other physiological processes that induce cell proliferation [2]. 5-BrdU can be probed by in vitro cell culture or in vivo injection and then specifically detected using anti-BdU antibodies [3]. After 5-BrdU labeling, an additional DNA denaturation step is required after fixation and permeabilization of tissues or cells to allow anti-5-BrdU antibodies to bind to 5-BrdU incorporated into DNA [4]. 5-BrdU antibodies can be used in combination with other cell markers such as Ki67, doublecortin (DCX) and NeuN to identify proliferating cells and newly differentiated neurons [5].
References:
[1] Zhu H. Cell proliferation assay by flow cytometry (BrdU and PI staining)[J]. Bio-protocol, 2012: e198-e198.
[2] Ziv Y, Ron N, Butovsky O, et al. Immune cells contribute to the maintenance of neurogenesis and spatial learning abilities in adulthood[J]. Nature neuroscience, 2006, 9(2): 268-275.
[3] Matiašová A, Ševc J, Mikeš J, et al. Flow cytometric determination of 5-bromo-2ʹ-deoxyuridine pharmacokinetics in blood serum after intraperitoneal administration to rats and mice[J]. Histochemistry and cell biology, 2014, 142: 703-712.
[4] Wolter S, Dittmar F, Seifert R. cCMP and cUMP in apoptosis: concepts and methods[J]. Non-canonical Cyclic Nucleotides, 2017: 25-47.
[5] Diaz F. Characterization of the Neurogenic Response to Apoptosis of Cortical Glutamatergic Neurons in the Adult Brain[M]. Yeshiva University, 2013.
5-溴-2’-脱氧尿苷（5-BrdU，5-bromo-2-deoxundine5-BrdU）是一种合成的胸苷溴化类似物，通常用于测定DNA合成和标记分裂细胞[1]。5-BrdU在S期可替代胸苷选择性插入细胞DNA，可以用来研究诱导细胞增殖的信号通路和其他生理过程[2]。5-BrdU可以通过体外细胞培养或体内注射的方式进行探针加载，然后用抗BdU的抗体进行特异性检测[3]。5-BrdU标记后，对组织或细胞进行固定和透化后，需要额外的DNA变性步骤，从而允许抗5-BrdU的抗体能够与插入DNA的5-BrdU结合[4]。5-BrdU抗体能够与其他细胞标记物如Ki67，双皮质素（DCX）和NeuN联合使用来鉴定增殖细胞和新分化神经元[5]。