Fetuin, Fetal Bovine Serum is a glycoprotein from fetal bovine serum, which is often an important supplement to many serum-free media. Bovine fetuin preparation has inhibited trypsin activity and promote cellular attachment, growth, and differentiation in many different culture systems [1]. Fetuins are established as a family within the cystatin superfamily [2].
Fetuin, Fetal Bovine Serum had activity on the S3 strain of HeLa cells for promoting cell attachment and stretching (flattening) on the glass [3]. Fetuin, Fetal Bovine Serum preparations stimulated the growth of a variety of mammalian cells from different species. Fetuin, Fetal Bovine Serum (30- 960 µg/ml) stimulated the growth of Hela cell [4]. The nuclei of the LNCaP cells that had been incubated with the Fetuin, Fetal Bovine Serum showed the characteristics of apoptosis [5].
Fetuin, Fetal Bovine Serum enhanced survival rate by up to 141% in the P388 leukemia model. In the PC-3 prostate cancer animal model, fetuin (50 mg/kg) seems to completely inhibit the formation of prostate cancer in mice [5].
References:
[1]. Nie Z. Fetuin: its enigmatic property of growth promotion[J]. American Journal of Physiology-Cell Physiology, 1992, 263(3): C551-C562.
[2]. Brown W M, Dziegielewska K M, Saunders N R, et al. Fetuin-an old friend revisited[J]. Bioessays, 1992, 14(11): 749-755.
[3]. Fisher H W, Puck T T, Sato G. Molecular growth requirements of single mammalian cells: The action of fetuin in promoting cell attachment to glass[J]. Proceedings of the National Academy of Sciences, 1958, 44(1): 4-10.
[4]. Marr A G M, Owen J A, Wilson G S. Studies on the growth-promoting glycoprotein fraction of foetal calf serum[J]. Biochimica et Biophysica Acta, 1962, 63(2): 276-285.
[5]. Yu C L, Tsai M H. Fetal fetuin selectively induces apoptosis in cancer cell lines and shows anti-cancer activity in tumor animal models[J]. Cancer letters, 2001, 166(2): 173-184.