Daphnoretin is a coumarin that has been found in W. indica and has diverse biological activities.1,2,3 It induces aggregation of washed rabbit platelets (EC50 = 17.2 μM), an effect that is reversed by the protein kinase C (PKC) inhibitor staurosporine .1 Daphnoretin is active against respiratory syncytial virus (RSV) in a plaque reduction assay using HEp-2 cells (IC50 = 5.87 μg/ml).2 It halts the cell cycle at the G2/M phase, induces apoptosis, and inhibits proliferation of human osteosarcoma (HOS) cells (IC50 = 3.89 μM).3 Daphnoretin also reduces proliferation, invasion, and migration of HCT116 colon cancer cells in a concentration-dependent manner.4
1.Feng, N.K.O., Chang, Y.L., Kuo, Y.H., et al.Daphnoretin, a new protein kinase C activator isolated from Wikstroemia indica C.A. MeyBiochem J.295(Pt 1)321-327(1993) 2.Ho, W.-S., Xue, J.-Y., Sun, S.S.M., et al.Antiviral activity of daphnoretin isolated from Wikstroemia indicaPhytother. Res.24(5)657-661(2010) 3.Gu, S., and He, J.Daphnoretin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cellsMolecules17(1)598-612(2012) 4.Yu, S., Guo, H., Gao, X., et al.Daphnoretin: An invasion inhibitor and apoptosis accelerator for colon cancer cells by regulating the Akt signal pathwayBiomed. Pharmacother.1111013-1021(2019)