Sodium palmitate is a common saturated fatty acid produced during the process of fatty acid synthesis. In the field of life science research, sodium palmitate is primarily used for studying cell metabolism, lipid research, and as a nutritional supplement in cell culture. Sodium palmitate can enhance lipogenesis and lipid droplet formation in various cell lines, and it is used to establish cell models of lipid droplet accumulation.
In vitro, Sodium palmitate (0.1, 0.25, or 0.5 mM; 12-72 h) can increase the mRNA levels of Notch1, -2, and -4 in LX2, Huh7, and MIHA liver cell lines[1]. Sodium palmitate can induce the expression of Glucose Regulated Protein 78 (GRP 78) and CCAAT/Enhancer Binding Protein Homologous Protein (CHOP) in mouse granule cells[2]. Moreover, treating LO2 cells with 100 µmol/l sodium palmitate for 48 hours significantly inhibits cell viability, and this concentration and incubation time can be used as a model of lipotoxicity in experiments[3].
References:
[1] Wen-Jin Ding, et al. Expression of Notch family is altered in non‑alcoholic fatty liver disease. Mol Med Rep. 2020 Sep;22(3):1702-1708.
[2] Harada H, et al. Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga. Anticancer Res. 2002 Sep-Oct;22(5):2587-90.
[3] Ma, Cheng, Yuan, et al. Lipid storage droplet protein 5 reduces sodium palmitateinduced lipotoxicity in human normal liver cells by regulating lipid metabolismrelated factors[J]. Molecular Medicine Reports, 2019, 20(2):879-886.
棕榈酸钠（Sodium palmitate）是一种常见的饱和脂肪酸，在脂肪酸合成过程中产生。棕榈酸钠在生命科学研究领域主要用于研究细胞代谢、脂质研究、以及作为细胞培养中的营养补充物。棕榈酸钠可增强各种细胞系中的脂肪生成和细胞脂肪变性，用于建立细胞脂肪变性模型。
在体外，棕榈酸钠(0.1、0.25 或 0.5 mM；12-72 h) 可增加 LX2、Huh7 和 MIHA 肝细胞系中Notch1、-2和-4的mRNA 水平[1]。棕榈酸钠可诱导小鼠颗粒细胞中葡萄糖调节蛋白78（GRP 78）和CCAAT/增强子结合蛋白同源蛋白（CHOP）的表达[2]。此外，用100 µmol/l棕榈酸钠处理LO2细胞48h，细胞活力显著受到抑制，该浓度和孵育时间可在实验中用作脂毒性模型[3]。