HOE 32021 is a Hoechst series of dyes that are commonly used for nuclear labeling staining of living cells. Hoechst dyes can be used to monitor cell viability by tracking changes in their emission spectra. As slightly groove-binding DNA stains with AT selectivity, Hoechst dyes are able to bind to all nucleic acids, but they show greater fluorescence enhancement for AT-rich double-stranded DNA strands compared to GC-rich strands [1]. This property has been used to identify Q bands in chromosomes, which are AT base pair-rich regions that fluoresce brightly when stained with quinacrine dye [2]. The fluorescence intensity of Hoechst dye increases as the pH of the solution increases.
References:
[1]. Karen E. Selph. Enumeration of marine microbial organisms by flow cytometry using near-UV excitation of Hoechst 34580-stained DNA.
[2]. Jonas Bucevičius, Gražvydas Lukinavičius, Rūta Gerasimaitė. The Use of Hoechst Dyes for DNA Staining and Beyond. Chemosensors 2018, 6(2), 18. Doi:org/10.3390/chemosensors6020018.
HOE 32021是Hoechst系列染料，常用于活细胞的细胞核标记染色。Hoechst染料可被用于通过跟踪其发射光谱的变化来监测细胞活力。作为具有AT选择性的轻微凹槽结合DNA染色剂，Hoechst染料能够与所有核酸结合，但与富含GC的链相比，它们对富含AT的双链DNA链显示出更大的荧光增强[1]。该特性已被用于鉴定染色体中的Q条带，这些染色体是富含AT碱基对的区域，当用奎纳克林染料染色时会发出明亮的荧光[2]。Hoechst染料的荧光强度随着溶液的pH的增加而增加。