GlpBio

DNA/RNA Synthesis

DNA/RNA Synthesis(DNA/RNA合成)

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis 相关产品(463)

  • GC33496 structure
    GC334962-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine)
    CAS: 10030-78-1
    纯度: >97.00%

    2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine) (N2-Methylguanine) 是一种修饰的核苷。

  • GC33577 structure
    GC335777-Aminoactinomycin D (7-AAD)
    CAS: 7240-37-1
    纯度: >95.00% / >97.00% / >98.00%

    7-Aminoactinomycin D是放线菌素D的荧光衍生物，选择性地与DNA的GC区域结合。

  • GC34090 structure
    GC34090Acelarin (NUC-1031)
    CAS: 840506-29-8
    纯度: >99.50%

    A prodrug form of gemcitabine

  • GC34123 structure
    GC34123Deoxycytidine triphosphate (dCTP)
    CAS: 2056-98-6

    脱氧胞苷三磷酸 (dCTP) (dCTP) 是一种核苷三磷酸，可用于 DNA 合成。

  • GC34452 structure
    GC344523,7,4'-Trihydroxyflavone
    CAS: 2034-65-3
    纯度: >98.00%

    3,7,4'-Trihydroxyflavone是从Rhusjavanicavar.roxburghiana中分离得到的一种具有DNA断链活性的黄酮类化合物。

  • GC35166 structure
    GC351665-Methylcytosine
    CAS: 554-01-8
    纯度: >99.50%

    5-Methylcytosine, a methylated form of the DNA base cytosine, is a major epigenetic mark in the nuclear DNA in mammals and may be involved in the regulation of gene transcription.

  • GC35247 structure
    GC35247ACX-362E
    CAS: 1275582-97-2
    纯度: >99.50%

    ACX-362E (ACX-362E) 是一流的、具有口服活性的 DNA 聚合酶 IIIC (pol IIIC) 抑制剂，Ki 为 0.325 μM 用于来自 C 的 DNA pol IIIC。

  • GC35761 structure
    GC35761CX-5461 dihydrochloride
    纯度: >98.00%

    A selective RNA polymerase inhibitor

  • GC35829 structure
    GC35829Dehydroaltenusin
    CAS: 31186-13-7

    Dehydroaltenusin 脱氢阿霉素是真核DNA 聚合酶 α (DNA polymerase α) 的小分子选择性抑制剂，一种由真菌产生的抗生素，其 IC50值为 0.68 μM。 Dehydroaltenusin 对哺乳动物 polα 活性的抑制作用模式对 DNA 模板引物 (Ki=0.23 μM) 是竞争性的，对 2'-脱氧核糖核苷 5'-三磷酸底物是非竞争性的 (Ki=0.18 μM)。Dehydroaltenusin 在 S 期阻止癌细胞周期并触发凋亡 apoptosis。Dehydroaltenusin 在体内具有抗人类腺癌肿瘤的抗肿瘤活性。

  • GC35888 structure
    GC35888DNA31
    CAS: 845626-57-5
    纯度: >98.00%

    DNA31 是囊性纤维化跨膜传导调节因子 (CFTR) 基因的全球最常见的 31 种突变。DNA31 用于针对囊性纤维化 (CF) 的新生儿筛查方案的第一级遗传测试。

  • GC36367 structure
    GC36367JH-RE-06
    CAS: 1361227-90-8
    纯度: >98.00%

    JH-RE-06 is a potent REV1-REV7 interface inhibitor with an IC50 of 0.78 μM and Kd value of 0.42 μM, disrupting REV1-POL ζ-mediated mutagenic translesion synthesis (TLS).

  • GC36743 structure
    GC36743Nimustine hydrochloride
    CAS: 55661-38-6
    纯度: >98.00%

    A pyrimidine analog and nitrosourea alkylating agent

  • GC37042 structure
    GC37042Pyrazofurin
    CAS: 30868-30-5
    纯度: >98.00%

    Pyrazofurin 是一种具有抗肿瘤活性的嘧啶核苷类似物，通过抑制尿苷5'-磷酸 (UMP) 合酶抑制细胞增殖和细胞内 DNA 合成。 Pyrazofurin 是一种活性，敏感的乳清酸-磷酸核糖基转移酶 (orotate-phosphoribosyltransferase) 抑制剂，在三种鳞状细胞癌 (SCC) 细胞系 Hep-2，HNSCC-14B 和 HNSCC-14C 的 IC50s 在 0.06-0.37 μM 之间。

  • GC37044 structure
    GC37044Pyrindamycin A
    CAS: 118292-36-7

    Pyrindamycin A 吡啶霉素 A 是一种抑制 DNA 合成的抗生素。 Pyrindamycin A 显示出抗鼠白血病的抗肿瘤活性，对小鼠和人肿瘤细胞系具有强烈的细胞毒活性，特别是对抗多柔比星抗性细胞。

  • GC37051 structure
    GC37051Quarfloxin
    CAS: 865311-47-3
    纯度: >99.50%

    Quarfloxin (CX-3543) 是一种具有抗肿瘤活性的氟喹诺酮类衍生物，其靶向抑制RNA pol I的活性，在神经母细胞瘤中的IC50 值在纳摩尔级别。Quarfloxin 干扰核糖体DNA中核蛋白与G-四重DNA结构的相互作用。

  • GC37521 structure
    GC37521RG7800 hydrochloride
    纯度: >99.50%

    RG7800 hydrochloride 是一种可口服的 SMN2 剪接调节剂，对 SMN2 剪接及 SMN 蛋白的 EC1.5x 值分别为 23 nM 和 87 nM。

  • GC37775 structure
    GC37775TH287 hydrochloride
    CAS: 1638211-05-8

    A selective MTH1 inhibitor

  • GC37777 structure
    GC37777TH588 hydrochloride
    CAS: 1640282-30-9

    TH588 hydrochloride是一种MTH1（NUDT1；IC 50 =5nM）抑制剂。

  • GC37940 structure
    GC37940Xanthopterin
    CAS: 119-44-8

    Xanthopterin，一种非共轭的蝶啶化合物，是东方大黄蜂翅膀中黄色颗粒的主要成分，在 386/456 nm 处产生特征性的激发光最大值。 Xanthopterin (XPT) 引起大鼠肾脏生长和肥大。Xanthopterin抑制 RNA 合成。

  • GC37941 structure
    GC37941Xanthopterin (hydrate)
    CAS: 5979-01-1
    纯度: >98.00%

    Xanthopterin, isolated from butterfly wings and found in many other sources, replace folic acid in the nutrition of many animal species.

  • GC38000 structure
    GC38000β-Boswellic acid
    CAS: 631-69-6
    纯度: >98.50%

    A pentacyclic triterpene with diverse bioactivities

  • GC38201 structure
    GC38201Metarrestin
    CAS: 1443414-10-5
    纯度: >99.50%

    Metarrestin (ML246) is an orally active and specific perinucleolar compartment (PNC) inhibitor that disrupts the nucleolar structure and inhibits RNA polymerase (Pol) I transcription. Metarrestin blocks metastatic development and extends survival in mouse cancer models.

  • GC38202 structure
    GC38202DTP3 TFA
    纯度: >98.50%

    DTP3 is a selective GADD45β/MKK7 (growth arrest and DNA-damage-inducible β/mitogen-activated protein kinase kinase 7) inhibitor and is able to restore MKK7/JNK activation. DTP3 inhibits cancer-selective NF-κB survival pathway.

  • GC38392 structure
    GC38392Euscaphic acid
    CAS: 53155-25-2

    A triterpene with diverse biological activities