GlpBio

DNA/RNA Synthesis

DNA/RNA Synthesis(DNA/RNA合成)

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis 相关产品(463)

  • GC38463 structure
    GC38463ASLAN003
    CAS: 1035688-66-4
    纯度: >99.50%

    A DHODH inhibitor

  • GC38466 structure
    GC38466Pseudouridimycin
    CAS: 1566586-52-4

    Pseudouridimycin (PUM) 是一种抗生素，选择性细菌 RNA 聚合酶 (RNAP) 抑制剂。Pseudouridimycin 是一种 C-核苷类似物，对革兰氏阴性菌和革兰氏阳性菌均有效。

  • GC38896 structure
    GC38896Caracemide
    CAS: 81424-67-1

    Caracemide (NSC-253272) 可以抑制大肠杆菌的核糖核苷酸还原酶。Caracemide是衍生自异羟肟酸的新型抗癌药，会产生严重的中枢神经系统 (CNS) 毒性。

  • GC39140 structure
    GC39140Isopimpinellin
    CAS: 482-27-9
    纯度: >98.00%

    A furanocoumarin with diverse biological activities

  • GC39149 structure
    GC39149D-I03
    CAS: 688342-78-1
    纯度: >99.50%

    D-I03 is a selective inhibitor of RAD52 with Kd of 25.8 ?M. D-I03 inhibits ssDNA annealing by RAD52 and D-loop formation with IC50 of 5 ?M and 8 ?M, respectively.

  • GC39234 structure
    GC39234DHODH-IN-2
    CAS: 1644156-80-8
    纯度: >99.50%

    DHODH-IN-2 是人二氢乳清酸脱氢酶 (HsDHODH) 抑制剂，可抑制麻疹病毒复制，pMIC50 值为 8.6。

  • GC39279 structure
    GC39279hDHODH-IN-1
    CAS: 1173715-42-8
    纯度: >99.50%

    hDHODH-IN-1 is a potent inhibitor of human dihydroorotate dehydrogenase (hDHODH) with anti-inflammatory effect.

  • GC39374 structure
    GC39374DHODH-IN-5
    CAS: 1644156-56-8
    纯度: >99.50%

    DHODH-IN-5 是一种有效的人二氢硼酸脱氢酶 (DHODH) 抑制剂，人重组 DHODH 的pIC50 为 7.8。DHODH-IN-5 抑制麻疹病毒复制，pMIC50 为 8.8。

  • GC39510 structure
    GC39510Synucleozid
    CAS: 502139-01-7

    Synucleozid (NSC 377363) 是可编码 α-突触核蛋白的 SNCA mRNA 的有效抑制剂 (IC50=1.5 μM)。Synucleozid 选择性靶向 α-synuclein mRNA 的 5'UTR 设计 IRE 位点，减少了加载到多核糖体中的 SNCA mRNA 的量，从而抑制了α-synuclein 蛋白的翻译。Synucleozid 有潜力用于帕金森病的相关研究。

  • GC39511 structure
    GC39511Synucleozid hydrochloride
    CAS: 2741856-68-6
    纯度: >98.00%

    Synucleozid (NSC 377363) 是可编码 α-突触核蛋白的 SNCA mRNA 的有效抑制剂 (IC50=1.5 μM)。Synucleozid 选择性靶向 α-synuclein mRNA 的 5'UTR的 IRE 位点，减少了加载到多核糖体中的 SNCA mRNA 的量，从而抑制了α-synuclein 蛋白的翻译。Synucleozid 有潜力用于帕金森病的相关研究。

  • GC39512 structure
    GC39512Tempo
    CAS: 2564-83-2

    Tempo 是一种经典的一氧化氮自由基，也 是一种线粒体 ROS 的选择性清除剂，可在催化循环中使超氧化物歧化。Tempo 可诱导 DNA 链断裂，并可用作将伯醇氧化为醛的有机催化剂。Tempo 具有诱变和抗氧化作用。

  • GC39527 structure
    GC395272-O-Methylcytosine
    CAS: 3289-47-2
    纯度: >95.00%

    2-O-Methylcytosine，一种 O-烷基化类似物和 DNA 的加合物，属于受损的核碱基。

  • GC39552 structure
    GC39552Sorivudine
    CAS: 77181-69-2
    纯度: >95.00%

    Sorivudine (BV-araU) 是一种口服活性的，合成的嘧啶核苷抗代谢 (pyrimidine nucleoside antimetabolite) 药物。Sorivudine 的抗病毒活性来自某些 DNA 病毒中存在的特定胸苷激酶 (thymidine kinase) 选择性转化为核苷酸，从而反过来会干扰病毒 DNA 合成 (DNA synthesis)。

  • GC39632 structure
    GC39632Laflunimus
    CAS: 147076-36-6
    纯度: >99.00%

    Laflunimus (HR325) 是一种免疫抑制剂和 Leflunomide 活性代谢物 A771726 的类似物。Laflunimus 是一种口服的二氢硼酸脱氢酶 (DHODH) 抑制剂。Laflunimus 抑制免疫球蛋白 (Ig) 分泌，抑制 Ig M 和 IgG 分泌的 IC50 值分别为 2.5 和 2 μM。Laflunimus 也是前列腺素内过氧化物合酶 (PGHS) -1 和 -2 抑制剂。

  • GC39811 structure
    GC39811FIT-039
    CAS: 1113044-49-7
    纯度: >98.00%

    A Cdk9 inhibitor

  • GC40005 structure
    GC40005Aurodox
    CAS: 12704-90-4
    纯度: 85%（mixture of tautomers）

    A polyketide antibiotic

  • GC40032 structure
    GC40032Antipain (hydrochloride)
    CAS: 37682-72-7
    纯度: >95.00%

    Antipain (hydrochloride)是一种可逆性蛋白酶抑制剂。

  • GC40103 structure
    GC40103Herboxidiene
    CAS: 142861-00-5
    纯度: >70.00%

    Herboxidiene 作为一种有效的抗肿瘤剂，可以靶向剪接体的 SF3B 亚基。

  • GC40618 structure
    GC406183',4',7-Trihydroxyisoflavone
    CAS: 485-63-2
    纯度: >98.00%

    A natural isoflavone

  • GC40641 structure
    GC406415'-Ethynyl-2'-deoxycytidine
    CAS: 69075-47-4
    纯度: >98.00%

    A nucleoside analog

  • GC40683 structure
    GC40683N-(2-hydroxyethyl)-Naphthalimide
    CAS: 5450-40-8
    纯度: >95.00%

    A fluorescent probe

  • GC41088 structure
    GC41088(6S)-Tetrahydrofolic Acid
    CAS: 71963-69-4
    纯度: >95.00%

    An active diastereomer

  • GC41598 structure
    GC41598Tyrocidine Complex
    CAS: 8011-61-8
    纯度: >95.00%

    An antibiotic complex

  • GC41633 structure
    GC41633(R)-Prunasin
    CAS: 99-18-3
    纯度: >95.00%

    A cyanogenic glucoside