GlpBio

Rebaudioside A

目录号: GN10011纯度: >98.00%同义词: 莱苞迪甙 A
Rebaudioside A（从甜叶菊叶片中提取）可抑制α-葡萄糖苷酶活性，IC50值为35.01μg/ml。
Rebaudioside A
Cas No.: 58543-16-1
规格价格库存数量操作
20mg¥1,880.00现货
1
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产品描述 Description

Rebaudioside A, from leaves of Stevia rebaudiana, inhibits α-glucosidase activity with IC50=35.01μg/ml[1]. Rebaudioside A has been used in studies related to hypertension treatment, diabetes management, and dental caries prevention[2].

In vitro, Rebaudioside A pretreatment at 1mM for 2h can significantly stimulate the release of glucagon-like peptide 1 (GLP-1) from STC-1 cells[3]. Treatment with 5.0μM Rebaudioside A for 24 hours significantly increased the expression of CYP3A29 mRNA in IPEC-J2 cells[4]. Rebaudioside A (10−6mol/L) incubation for 60 minutes significantly increased insulin secretion in isolated mouse pancreatic islets stimulated by glucose at 16.7mM[5].

In vivo, Rebaudioside A (20mg/kg/day; administered twice daily; i.p.) treatment for 8 weeks can prevent liver damage caused by the administration of thioacetamide (200mg/kg; administered three times weekly, i.p.), and maintain collagen content in Wistar rats[6]. Oral administration of Rebaudioside A and Honokiol micelles (100mg/kg/day) for 15 consecutive days can inhibit tumor growth in the H22 solid tumor model of mice, without affecting the body weight of the mice[7].

References:
[1] Adari B R, Alavala S, George S A, et al. Synthesis of rebaudioside-A by enzymatic transglycosylation of stevioside present in the leaves of Stevia rebaudiana Bertoni[J]. Food chemistry, 2016, 200: 154-158.
[2] Wang Y, Luo X, Chen L, et al. Natural and low‐caloric rebaudioside A as a substitute for dietary sugars: A comprehensive review[J]. Comprehensive Reviews in Food Science and Food Safety, 2023, 22(1): 615-642.
[3] Noya-Leal F, van der Wielen N, Behrens M, et al. Rebaudioside A from Stevia rebaudiana stimulates GLP-1 release by enteroendocrine cells via bitter taste signalling pathways[J]. Food & Function, 2023, 14(15): 6914-6928.
[4] Thøgersen R, Petrat-Melin B, Zamaratskaia G, et al. In vitro effects of rebaudioside A, stevioside and steviol on porcine cytochrome p450 expression and activity[J]. Food Chemistry, 2018, 258: 245-253.
[5] Abudula R, Jeppesen P B, Rolfsen S E D, et al. Rebaudioside A potently stimulates insulin secretion from isolated mouse islets: studies on the dose-, glucose-, and calcium-dependency[J]. Metabolism, 2004, 53(10): 1378-1381.
[6] Casas‐Grajales S, Reyes‐Gordillo K, Cerda‐García‐Rojas C M, et al. Rebaudioside A administration prevents experimental liver fibrosis: An in vivo and in vitro study of the mechanisms of action involved[J]. Journal of Applied Toxicology, 2019, 39(8): 1118-1131.
[7] Wang J, Yang H, Li Q, et al. Novel nanomicelles based on rebaudioside A: a potential nanoplatform for oral delivery of honokiol with enhanced oral bioavailability and antitumor activity[J]. International journal of pharmaceutics, 2020, 590: 119899.

Rebaudioside A（从甜叶菊叶片中提取）可抑制α-葡萄糖苷酶活性，IC50值为35.01μg/ml[1]。Rebaudioside A已被应用于高血压治疗、糖尿病调控及龋齿防治等研究领域[2]。

在体外，1mM浓度的Rebaudioside A预处理STC-1细胞2小时，可显著促进胰高血糖素样肽-1（GLP-1）的释放[3]。5.0μM浓度的Rebaudioside A处理IPEC-J2细胞24小时，能显著提升CYP3A29 mRNA表达水平[4]。10−6mol/L浓度的Rebaudioside A孵育60分钟，可显著增强由16.7mM葡萄糖刺激的离体小鼠胰岛的胰岛素分泌[5]。

在体内，Rebaudioside A（20mg/kg/day，每日两次腹腔注射）持续处理8周，可预防硫代乙酰胺（200mg/kg，每周三次腹腔注射）诱导的Wistar大鼠肝损伤并维持胶原蛋白含量[6]。连续15天口服Rebaudioside A与和Honokiol胶束（100mg/kg/day），能抑制H22实体瘤小鼠模型的肿瘤生长，且不影响小鼠体重[7]。

实验参考方法 Experimental Reference Method

Cell experiment [1]:

Cell lines

IPEC-J2 cells

Preparation Method

The IPEC-J2 cells were cultured in the modified Eagle's medium/Ham's F-12 medium (DMEM/F-12) supplemented with 100 units/ml penicillin, 100µg/ml streptomycin and 10% heat-inactivated fetal bovine serum (FBS). The cells were cultured at 37°C, 5% CO2 and 95% relative humidity, and the medium was changed three times a week. The cells were cultured in 25 or 75cm2 simple culture flasks and passaged before confluence (approximately every 3-4 days). The IPEC-J2 cells were cultured in the transwell chambers and treated with the medium containing Rebaudioside A (0.5, 2.5, 5.0μM). The cells were incubated for 24 hours, washed with PBS, and 800µl of Tri-Reagent was added for extracting RNA. cDNA was synthesized using a cDNA synthesis kit, and real-time PCR was performed under the following conditions: 50°C for 2 minutes, 95°C for 10 minutes, followed by 40 cycles of 15 seconds at 95°C and 1 minute at 60°C. The relative mRNA expression was standardized based on the mRNA expression of β-actin and reported as the relative mRNA expression compared to the control. Cell viability was determined using the MTT method. The cells were washed with PBS, then 0.5mg/ml MTT was added to the culture medium. After 2.5 hours of incubation, PBS was used to wash the cells, and 300µl of DMSO was added to dissolve the formed methylene blue crystals. After incubation at room temperature for 15 minutes, 100µl was transferred to a transparent 96-well microtiter plate, and the absorbance was measured at 590nm.

Reaction Conditions

0.5, 2.5, 5.0μM; 24h

Applications

Rebaudioside A induced a significant increase in intracellular CYP3A29 expression in a dose-dependent manner, without affecting cell viability.
Animal experiment [2]:

Animal models

Wistar rats

Preparation Method

Place 100-120g, 1-month-old male Wistar rats in polycarbonate cages under controlled conditions (21±1°C, 50%-60% relative humidity, and a 12-hour dark/light cycle). Feed them with rat food and provide water freely. Divide the rats into four groups (n = 8). Generate liver fibrosis by intraperitoneal injection of thioacetamide (TAA) three times a week (200mg/kg; i.p.) for 8 weeks. During TAA treatment, inject Rebaudioside A twice a day (20mg/kg; i.p.). The first group (n = 8) consisted of control animals that only received the carrier (saline, i.p.); the second group (n = 8) received TAA; the third group (n = 8) received TAA dissolved in saline solution intraperitoneally daily; the fourth group (n = 8) only received Rebaudioside A twice a day (20mg/kg; i.p.). Under mild ketamine/salazopyrin anesthesia, bleed the animals through cardiac puncture and quickly obtain the liver and blood samples. Take liver sections from the right lobe of each rat and store them at -70°C for further immunohistochemical assays.

Dosage form

20mg/kg, twice a day for 8 weeks a day; i.p.

Applications

Rebaudioside A treatment blocked the oxidation process by upregulating nuclear erythropoietin 2, thereby preventing liver damage in Wistar rats.

References:
[1] Thøgersen R, Petrat-Melin B, Zamaratskaia G, et al. In vitro effects of rebaudioside A, stevioside and steviol on porcine cytochrome p450 expression and activity[J]. Food Chemistry, 2018, 258: 245-253.
[2] Casas?Grajales S, Reyes?Gordillo K, Cerda?García?Rojas C M, et al. Rebaudioside A administration prevents experimental liver fibrosis: An in vivo and in vitro study of the mechanisms of action involved[J]. Journal of Applied Toxicology, 2019, 39(8): 1118-1131.

产品文档 Product Documents

Purity:>98.00%

化学性质Chemical Properties

CAS 号
58543-16-1
同义词
莱苞迪甙 A
化学名
Rebiana; Stevioside a3; Sweetener 4G-S
SMILES
O[C@@H]1[C@@H](CO)O[C@@]([H])(OC2C[C@]([C@@](CCC[C@@]3(C)C(OC4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)=O)(C)[C@]3([H])CC5)([H])C5(C6)CC2C6=C)[C@H](O[C@@]7([H])[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O7)C1O[C@@]8([H])[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O8
分子式
C44H70O23
分子量
967.1 g/mol
溶解性
DMF: 1 mg/ml,DMSO: 1 mg/ml,PBS (pH 7.2): 10 mg/ml
保存条件
Store at 2-8°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

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