17(S)-HDHA-d5 is intended for use as an internal standard for the quantification of 17(S)-HDHA by GC- or LC-MS. 17(S)-HDHA is a hydroxy fatty acid formed from docosahexaenoic acid by 15-lipoxygenase (15-LO) and is a precursor to 17(S)-resolvins.1,2 17(S)-HDHA inhibits platelet 12-LO (IC50 = 0.4 μM).1 It inhibits TNF-α-induced expression of IL1B in a human glial cell line (IC50 = ~0.5 nM).2 17(S)-HDHA (100 nM) inhibits NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome formation induced by homocysteine in podocytes.3
|1. Mitchell, P.D., Hallam, C., Hemsley, P.E., et al. Inhibition of platelet 12-lipoxygenase by hydroxy-fatty acids. Biochem. Soc. Trans. 12, 839-841 (1984).|2. Hong, S., Gronert, K., Devchand, P.R., et al. Novel docosatrienes and 17S-resolvins generated from docosahexaenoic acid in murine brain, human blood, and glial cells. Autacoids in anti-inflammation. J. Biol. Chem. 278(17), 14677-14687 (2003).|3. Li, G., Chen, Z., Bhat, O.M., et al. NLRP3 inflammasome as a novel target for docosahexaenoic acid metabolites to abrogate glomerular injury. J. Lipid Res. 58(6), 1080-1090 (2017).