Ecdysterone is a naturally occurring ecdysone that controls molting (feathering) and metamorphosis in arthropods[1]. Ecdysterone can promote protein synthesis in skeletal muscle cells[2]. Ecdysterone can be used as a sports nutrition supplement and in metabolic disease research[3, 4].
In vitro, Ecdysterone (0-500μM) treatment of MCF7, MDA-MB-231, and MDA-MB-468 cells for 96h inhibited colony formation, cell proliferation, and induced cell death in the three cell lines[5]. Ecdysterone (1, 50, 100μM) pretreatment of PC12 cells for 24h reversed the cytotoxic effects induced by β-amyloid peptide fragment 25-35 (Aβ25-35) in a dose-dependent manner[6].
In vivo, subcutaneous injection of Ecdysterone (0.6, 0.8, 1mg/kg) in osteoarthritis (OA) rats for 4 weeks significantly improved the pathological state of cartilage tissue, inhibited inflammation in cartilage tissue, and upregulated the expression levels of autophagy markers LC3II/LC3I in cartilage tissue[7].
References:
[1] Lafont R, Balducci C, Dinan L. Ecdysteroids[J]. Encyclopedia, 2021, 1(4): 1267-1302.
[2] Gorelick-Feldman J, MacLean D, Ilic N, et al. Phytoecdysteroids increase protein synthesis in skeletal muscle cells[J]. Journal of agricultural and food chemistry, 2008, 56(10): 3532-3537.
[3] Isenmann E, Ambrosio G, Joseph J F, et al. Ecdysteroids as non-conventional anabolic agent: performance enhancement by ecdysterone supplementation in humans[J]. Archives of toxicology, 2019, 93(7): 1807-1816.
[4] Todorova V, Ivanova S, Chakarov D, et al. Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition[J]. Nutrients, 2024, 16(9): 1382.
[5] Shuvalov O, Fedorova O, Tananykina E, et al. An arthropod hormone, ecdysterone, inhibits the growth of breast cancer cells via different mechanisms[J]. Frontiers in Pharmacology, 2020, 11: 561537.
[6] Su-fen Y, Zhong-jun W, Zheng-qin Y, et al. Protective effect of ecdysterone on PC12 cells cytotoxicity induced by Beta-amyloid 25–35[J]. Chinese journal of integrative medicine, 2005, 11: 293-296.
[7] Tang Y, Mo Y, Xin D, et al. β-ecdysterone alleviates osteoarthritis by activating autophagy in chondrocytes through regulating PI3K/AKT/mTOR signal pathway[J]. American Journal of Translational Research, 2020, 12(11): 7174.
Ecdysterone是一种天然存在的蜕皮激素，能够控制节肢动物的蜕皮（换羽）和变态[1]。Ecdysterone能够促进骨骼肌细胞中的蛋白质合成[2]。Ecdysterone能够用于运动营养补充剂和代谢性疾病研究[3, 4]。
在体外，Ecdysterone（0-500μM）处理MCF7、MDA-MB-231、MDA-MB-468细胞96h，抑制了三种细胞系的集落形成，抑制了细胞增殖并诱导死亡[5]。Ecdysterone（1, 50, 100μM）预处理PC12细胞24h，以剂量依赖性方式逆转了β-淀粉样蛋白肽片段25-35（Aβ25-35）诱导的细胞毒性作用[6]。
在体内，Ecdysterone（0.6, 0.8, 1mg/kg）通过皮下注射治疗骨关节炎（OA）大鼠4周，显著改善了软骨组织的病理状态，抑制了软骨组织中的炎症，上调了软骨组织中自噬标志物LC3II/LC3I的表达水平[7]。