Bromoenol lactone-d7

Bromoenol lactone-d₇ (BEL-d₇) contains seven deuterium atoms at the 2, 3, 4, 5, 6, 7, and 8 positions. It is intended for use as an internal standard for the quantification of BEL by GC- or LC-mass spectrometry (MS). BEL is a selective, potent, irreversible, mechanism-based inhibitor of phospholipase A₂ (iPLA₂) with a K_i value of 180 nM.¹ It also inhibits macrophage iPLA₂ in a concentration-dependent manner with an IC₅₀ value of 60 nM and is an effective enzyme-activated irreversible inhibitor of chymotrypsin (K_i = 636 nM).^2,3

1.Hazen, S.L., Zupan, L.A., Weiss, R.H., et al.Suicide inhibition of canine myocardial cytosolic calcium-independent phospholipase A2The Journal of Biological Chemisty2667227-7232(1991) 2.Ackermann, E.J., Conde-Frieboes, K., and Dennis, E.A.Inhibition of macrophage Ca2+-independent phospholipase A2 by bromoenol lactone and trifluoromethyl ketonesThe Journal of Biological Chemisty270445-450(1995) 3.Balsinde, J., Bianco, I.D., Ackermann, E.J., et al.Inhibition of calcium-independent phospholipase A2 prevents arachidonic acid incorporation and phospholipid remodeling in P388D1 macrophagesProceedings of the National Academy of Sciences of the United States of America928527-8531(1995)