Brassinolide is a plant sterol first isolated from pollen of rape (Brassica napus L.) and play major roles in plant growth, development, and stress tolerance[1]. Brassinolide treatment in plants under salt stress conditions significantly increases the activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), which help in scavenging reactive oxygen species (ROS) and reducing oxidative damage[2].
In vitro, Brassinolide treatment (0, 10, 20 and 40μM) for 12, 24 and 36h can induce a time and concentration-dependent cytotoxicity in human prostate cancer PC-3 cells by increasing Caspase-3 activity and decreasing the expression of anti-apoptotic protein Bcl-2[3]. Brassinolide treatment (0.001-10μg/mL) could effectively reverse the resistance of human T lymphoblastoid cell line CCRF-VCR 1000 by inhibiting the effusion of drug transported by P-glucoprotein. To down regulate the abnormal expression of p53 maybe one of the mechanisms of reversing multidrug resistance for Brassinolide[4].
In vivo, diabetes rats induced by Alloxan orally administratead with Brassinolide at 50, 100 and 200mg/kg respectively has the function of reducing the blood glucose levels but not in a dose-dependent manner. The levels of blood glucose displayed significant differences after treatment with different dose of Brassinolide[5].
References:
[1] Vriet C, Russinova E, Reuzeau C. et al. From squalene to brassinolide: the steroid metabolic and signaling pathways across the plant kingdom. Mol Plant. 2013 Nov;6(6):1738-57.
[2] Hu Y Q, Xia S T, Su Y, et al. Brassinolide Increases Potato Root Growth In Vitro in a Dose-Dependent Way and Alleviates Salinity Stress. Biomed Res Int. 2016:2016:8231873.
[3]Wu YD, Lou Y J, et al. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells. Pharmazie. 2007 May;62(5):392-5.
[4] Xian LJ, et al. Reversing effect of brassinolide on multidrug resistance of-CCRF-VCR1000 cells and a preliminary investigation on its mechanisms. Yao Xue Xue Bao. 2005. Feb;40(2):117-2
[5] Chen S, He J, Wang Q, et al. Effect of Brassinolide on Levels of Blood Glucose in Alloxan—induced Diabetes Rats. Laboratory Animal Science, 2009, 3: 007
Brassinolide是一种植物甾醇，最初从油菜（Brassica napus L.）花粉中分离出来，在植物生长、发育和逆境耐受性方面发挥重要作用[1]。在盐胁迫条件下，Brassinolide处理显著提高了植物中超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）等抗氧化酶的活性，这些酶有助于清除活性氧（ROS）并减少氧化损伤[2]。
在体外实验中，Brassinolide处理（0、10、20和40μM）对人前列腺癌PC-3细胞进行12、24和36小时处理，可以诱导时间和浓度依赖性的细胞毒性，通过增加Caspase-3活性和降低抗凋亡蛋白Bcl-2的表达来实现[3]。Brassinolide处理（0.001-10μg/mL）能够有效逆转人T淋巴母细胞系CCRF-VCR 1000的耐药性，通过抑制由P-糖蛋白运输的药物外排来实现。下调p53的异常表达可能是Brassinolide逆转多药耐药性的一种机制[4]。
在体内实验中，通过Alloxan诱导的糖尿病大鼠口服给予Brassinolide（50, 100和200mg/kg）具有降低血糖水平的功能，但并非以剂量依赖性方式。不同剂量的Brassinolide处理后，血糖水平显示出显著差异[5]。