α-Factor Mating Pheromone, yeast (Mating Factor α) is a 13-amino acid peptide hormone that is continuously synthesized and secreted by MATα-type cells of Saccharomyces cerevisiae and can specifically act on MATa-type cells [1]. α-Factor Mating Pheromone, yeast (Mating Factor α) binds to the G protein-coupled receptor Ste2p on the surface of MATa cells, triggering cell cycle arrest, morphological changes, and the expression of mating-related genes. This factor can compete with radiolabeled ³⁵S-α-factor for binding (Kd = 0.3μM). The binding of α-Factor Mating Pheromone, yeast (Mating Factor α) to the thermosensitive ste2 mutant is thermally unstable, while the binding to other mutants (ste4, ste5, ste7, ste11 and ste12) is not thermally unstable [2]. α-Factor Mating Pheromone, yeast (Mating Factor α) is often used to induce yeast to enter a mating state and is widely used in the secretion and expression of foreign proteins [3].
References:
[1]. Naider F, Becker JM. The α-factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein-coupled receptors. Peptides. 2004 Sep 1; 25(9): 1441-1463.
[2]. Jenness DD, Burkholder AC, Hartwell LH. Binding of α-factor pheromone to yeast a cells: chemical and genetic evidence for an α-factor receptor. Cell. 1983 Dec 1; 35(2): 521-529.
[3]. Sieber B, Coronas-Serna JM, Martin SG. A focus on yeast mating: From pheromone signaling to cell-cell fusion. InSeminars in Cell & Developmental Biology 2023 Jan 15; 133: 83-95.
α-Factor Mating Pheromone, yeast (Mating Factor α)是一种由13个氨基酸组成的肽类激素，由酿酒酵母的MATα型细胞持续合成和分泌，并能特异性地作用于MATa型细胞 [1]。α-Factor Mating Pheromone, yeast (Mating Factor α)可与MATa细胞表面的G蛋白偶联受体Ste2p结合，从而引发细胞周期停滞、形态学变化以及交配相关基因的表达。该因子可与放射性标记的³⁵S-α-factor竞争结合（Kd = 0.3μM）。α-Factor Mating Pheromone, yeast (Mating Factor α)与温敏性ste2突变体的结合具有热稳定性，而与其他突变体（ste4、ste5、ste7、ste11和ste12）的结合则不具有热稳定性 [2]。α-Factor Mating Pheromone, yeast (Mating Factor α)常用于诱导酵母进入交配状态，并广泛应用于外源蛋白的分泌和表达 [3]。