Adenosine 5'-(γ-thio)-triphosphate (lithium salt) is a stable analog of ATP that acts as a potent agonist of G protein-coupled P2Y2 and P2Y11 receptors (pEC50 = 5.52 for P2Y11).[1] It has been used to identify kinase substrates, has been implemented as a reagent in the synthesis of DNA N-acetylglucosamine analogs, and can serve as a substrate for the RNA-stimulated nucleotide hydrolysis and RNA unwinding activities of eukaryotic initiation factor-4A.[2],[3],[4]
腺苷5'-（γ-硫代）-三磷酸（锂盐）是ATP的稳定类似物，作为G蛋白偶联的P2Y2和P2Y11受体的有效激动剂（P2Y11的pEC50=5.52）。[1] 它已被用于鉴定激酶底物，已被用作合成DNA N-乙酰葡糖胺类似物的试剂，并可作为真核起始因子-4A的RNA刺激的核苷酸水解和RNA解旋活性的底物。[2] ，[3]，[4]
Reference:
[1]. Jacobson, K.A., Ivanov, A.A., de Castro, S., et al. Development of selective agonists and antagonists of P2Y receptors. Purinergic Signal. 5(1), 75-89 (2009).
[2]. Knowles, J.R. Enzyme-catalyzed phosphoryl transfer reactions. Annual Reviews of Biochemistry 49, 877-919 (1980).
[3]. Chrysogelos, S., Register, J.C., III, and Griffith, J. The structure of recA protein-DNA filaments. 2 recA protein monomers unwind 17 base pairs of DNA by 11.5 degrees/base pair in the presence of adenosine 5'-O-(3-thiotriphosphate). The Journal of Biological Chemisty 258(20), 12624-12631 (1983).
[4]. Peck, M.L., and Herschlag, D. Adenosine 5'-O-(3-thio)triphosphate (ATPγS) is a substrate for the nucleotide hydrolysis and RNA unwinding activities of eukaryotic translation initiation factor elF4A. RNA 9(10), 1180-1187 (2003).