Adenosine 5′-diphosphoribose sodium is a nucleotide derivative and an agonist of Transient receptor potential melastatin 2 (TRPM2), playing an important role in various metabolic pathways within cells[1]. Adenosine 5′-diphosphoribose sodium can modulate intracellular signaling pathways, affecting processes such as cell proliferation, differentiation, and apoptosis[2]. Adenosine 5′-diphosphoribose sodium also plays a key role in cellular stress responses, helping cells cope with adverse conditions like oxidative stress[3]. Adenosine 5′-diphosphoribose sodium is closely related to cellular immune responses, participating in the regulation of immune cell activity[4].
In vitro, treatment of RIN-5F cells with Adenosine 5′-diphosphoribose sodium (100μM) increases intracellular calcium ion (Ca²⁺) concentration through the PLC-IP3 pathway[5]. Treatment of microglial cells with Adenosine 5′-diphosphoribose sodium (1mM) induces Ca²⁺ and Mg²⁺ influx[6].
References:
[1] Fliegert R, Bauche A, Wolf Pérez AM, et al. 2'-Deoxyadenosine 5'-diphosphoribose is an endogenous TRPM2 superagonist. Nat Chem Biol. 2017 Sep;13(9):1036-1044.
[2] Vyas S, Chang P. New PARP targets for cancer therapy. Nat Rev Cancer. 2014 Jul;14(7):502-9.
[3] Dousa TP, Chini EN, Beers KW. Adenine nucleotide diphosphates: emerging second messengers acting via intracellular Ca2+ release. Am J Physiol. 1996 Oct;271(4 Pt 1):C1007-24.
[4] Ji D, Luo ZW, Ovcjak A, et al. Role of TRPM2 in brain tumours and potential as a drug target. Acta Pharmacol Sin. 2022 Apr;43(4):759-770.
[5] Ishii M, Shimizu S, Hagiwara T, et al. Extracellular-added ADP-ribose increases intracellular free Ca2+ concentration through Ca2+ release from stores, but not through TRPM2-mediated Ca2+ entry, in rat beta-cell line RIN-5F. J Pharmacol Sci. 2006 Jun;101(2):174-8.
[6] Kraft R, Grimm C, Grosse K, et al. Hydrogen peroxide and ADP-ribose induce TRPM2-mediated calcium influx and cation currents in microglia. Am J Physiol Cell Physiol. 2004 Jan;286(1):C129-37.
Adenosine 5′-diphosphoribose sodium是一种核苷酸衍生物，是Transient receptor potential melastatin 2 (TRPM2)激动剂，在细胞内多种代谢途径中发挥着重要作用[1]。Adenosine 5′-diphosphoribose sodium能够调节细胞内的信号传导通路，影响细胞的增殖、分化和凋亡等过程[2]。Adenosine 5′-diphosphoribose sodium在细胞的应激反应中也扮演着关键角色，帮助细胞应对氧化应激等不利环境[3]。Adenosine 5′-diphosphoribose sodium还与细胞的免疫反应密切相关，参与调节免疫细胞的活性[4]。
在体外，Adenosine 5′-diphosphoribose sodium（100μM）处理RIN-5F细胞，Adenosine 5′-diphosphoribose sodium通过PLC- IP3途径增加细胞内钙离子（Ca²⁺）浓度[5]。Adenosine 5′-diphosphoribose sodium（1mM）处理小胶质细胞，可诱导Ca²⁺内流和Mg²⁺内流[6]。