Tautomycin, an antifungal antibiotic isolated from the bacterium Streptomyces verticillatus, is a potent and specific inhibitor of protein phosphatases 1 and 2A and induces contraction of smooth muscle under Ca2+-free conditions, with Kiapp values of 0.16 nM and 0.4 nM for PP1 and PP2A, respectively[1][2].
MTC cell growth is inhibited in a dose-dependent manner by Tautomycin (0-1000 nM, 48 hours)[2].NE markers ASCL1 and CgA in human MTC cells are decreased in a dose-dependent manner by Tautomycin (0-1000 nM, 48 hours)[2].|| Cell Proliferation Assay[2]||Cell Line:|Human MTC cells|Concentration:|0-1000 nM|Incubation Time:|48 hours|Result:|Inhibited MTC cell growth in a dose-dependent manner.|| Western Blot Analysis[2]||Cell Line:|Human MTC cells|Concentration:|0-1000 nM|Incubation Time:|48 hours|Result:|Led to a dose-dependent decrease in the NE markers ASCL1 and CgA.
[1]. MacKintosh C, et al. Tautomycin from the bacterium Streptomyces verticillatus. Another potent and specific inhibitor of protein phosphatases 1 and 2A. FEBS Lett. 1990 Dec 17;277(1-2):137-40. [2]. Adler JT, et al. Tautomycetin and tautomycin suppress the growth of medullary thyroid cancer cells via inhibition of glycogen synthase kinase-3beta. Mol Cancer Ther. 2009 Apr;8(4):914-20.