Triclosan is a broad-spectrum antibacterial agent[1]. Triclosan inhibits bacterial fatty acid synthesis at the enoyl-acyl carrier protein reductase (FabI) step[2]. Triclosan is commonly used for antibacterial research and is widely found in daily necessities such as soaps, detergents, toys, and surgical cleaning treatments[3][4].
In vitro, Triclosan (1-100µM; 1-24h) decreased cell viability and induced cell apoptosis in a dose- and time-dependent manner in rat neural stem cells (NSCs)[5].
In vivo, Triclosan (50-200mg/kg/day; p.o.; 7 days) significantly reduced serum testosterone levels, downregulated the expression of Leydig cell genes involved in testosterone biosynthesis in adult male rats[6]. Triclosan (80ppm in diet; 4 weeks) increased the severity of dextran sodium sulfate (DSS)-induced colitis, and promoted colonic inflammation and immune cell infiltration in mice[7].
References:
[1] Vosatka R, Kratky M, Vinsova J. Triclosan and its derivatives as antimycobacterial active agents. Eur J Pharm Sci. 2018;114:318-331.
[2] Heath RJ, Rubin JR, Holland DR, Zhang E, Snow ME, Rock CO. Mechanism of triclosan inhibition of bacterial fatty acid synthesis. J Biol Chem. 1999;274(16):11110-11114.
[3] Shrestha P, Zhang Y, Chen WJ, Wong TY. Triclosan: antimicrobial mechanisms, antibiotics interactions, clinical applications, and human health. J Environ Sci Health C Toxicol Carcinog. 2020;38(3):245-268.
[4] Weatherly LM, Gosse JA. Triclosan exposure, transformation, and human health effects. J Toxicol Environ Health B Crit Rev. 2017;20(8):447-469.
[5] Park BK, Gonzales EL, Yang SM, Bang M, Choi CS, Shin CY. Effects of Triclosan on Neural Stem Cell Viability and Survival. Biomol Ther (Seoul). 2016;24(1):99-107.
[6] Sang J, Ji Z, Li H, et al. Triclosan inhibits testosterone biosynthesis in adult rats via inducing m6A methylation-mediated autophagy. Environ Int. 2024;190:108827.
[7] Zhang J, Walker ME, Sanidad KZ, et al. Microbial enzymes induce colitis by reactivating triclosan in the mouse gastrointestinal tract. Nat Commun. 2022;13(1):136.
Triclosan 是一种广谱抗菌剂[1]。Triclosan 抑制细菌脂肪酸在烯酰基载体蛋白还原酶 (FabI) 阶段的合成[2]。Triclosan 常用于抗菌研究，并广泛存在于肥皂、洗涤剂、玩具和外科清洁治疗等日用品中[3][4]。
体外实验中，Triclosan（1-100µM；1-24小时）以剂量和时间依赖性方式降低大鼠神经干细胞（NSCs）的细胞活力，并诱导细胞凋亡[5]。
体内实验中，Triclosan（50-200mg/kg/天；口服；7天）显著降低了成年雄性大鼠的血清睾酮水平，并下调了睾丸间质细胞中与睾酮生物合成相关的基因表达[6]。Triclosan（饮食中 80ppm；4周）加剧了小鼠葡聚糖硫酸钠（DSS）诱导的结肠炎，促进了结肠炎症和免疫细胞浸润[7]。