TRFS-green is a turn-on green fluorescent probe with high selectivity for thioredoxin reductase (TrxR). TRFS-green is widely used for imaging TrxR activity in live cells, with Ex/Em of 377nm/480nm, and 438nm/538nm under simulated physiological conditions[1]. TrxR is often overexpressed in many cancer cells, and its ablation can reverse the growth of various malignancies, reinforcing this selenoenzyme as a valid target for the development of novel anticancer drugs[2,3,4]. TRFS-green can facilitate the discovery of TrxR-targeting small molecules and advance the understanding of the physiological and pathological functions of TrxR in vivo[1,5].
References:
[1] ZHANG L, DUAN D, LIU Y, et al. Highly selective off–on fluorescent probe for imaging thioredoxin reductase in living cells[J]. Journal of the American Chemical Society, 2014, 136(1): 226-233.
[2] CAI W, ZHANG L, SONG Y, et al. Small molecule inhibitors of mammalian thioredoxin reductase[J]. Free Radical Biology and Medicine, 2012, 52(2): 257-265.
[3] POWIS G, KIRKPATRICK D L. Thioredoxin signaling as a target for cancer therapy[J]. Current Opinion in Pharmacology, 2007, 7(4): 392-397.
[4] HOLMGREN A, LU J. Thioredoxin and thioredoxin reductase: current research with special reference to human disease[J]. Biochemical and Biophysical Research Communications, 2010, 396(1): 120-124.
[5] ZHANG B, ZHANG J, PENG S, et al. Thioredoxin reductase inhibitors: a patent review[J]. Expert Opinion on Therapeutic Patents, 2017, 27(5): 547-556.
TRFS-green是一种对硫氧还蛋白还原酶（TrxR）具有较高选择性的“关-开”型绿色荧光探针，被广泛用于活细胞中TrxR活性的成像，Ex/Em为377nm/480nm，在模拟生理条件下为438nm/538nm[1]。TrxR常在许多癌细胞中过量表达，针对其消融可逆转多种恶性肿瘤的生长，增强该硒酶作为新型抗癌药物开发的有效靶点[2,3,4]。TRFS-green可促进对TrxR靶向小分子的发现，并推动对TrxR体内生理和病理功能的理解[1,5]。