GlpBio

DNA/RNA Synthesis

DNA/RNA Synthesis(DNA/RNA合成)

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis 相关产品(463)

  • GC50572 structure
    GC50572BC-LI-0186
    CAS: 695207-56-8
    纯度: >98.00% / >99.00%

    BC-LI-0186是一种有效、高选择性的亮氨酰-tRNA合成酶（LRS）与Ras相关GTP结合蛋白D（RagD）相互作用的抑制剂，IC 50 值为46.11nM。

  • GC50656 structure
    GC50656BAY 707
    CAS: 2109805-96-9
    纯度: >98.00%

    BAY 707 是一种底物竞争性、高效和选择性的 MTH1(NUDT1) 抑制剂，IC50 为 2.3 nM。 BAY 707 与其他 MTH1 化合物相比具有良好的药代动力学 (PK) 特征，并且在小鼠中具有良好的耐受性，但明显缺乏体外或体内抗癌功效。

  • GC52006 structure
    GC52006CB-096
    CAS: 108883-90-5
    纯度: >98.00%

    An inhibitor of RAN translation

  • GC52165 structure
    GC52165Minosaminomycin
    CAS: 51746-09-9
    纯度: >90.00%

    An antibiotic

  • GC52171 structure
    GC52171Clindamycin-d3 (hydrochloride)
    CAS: 1356933-72-6
    纯度: >99.00%

    An internal standard for the quantification of clindamycin

  • GC52183 structure
    GC521832'-O-Methyl-5-methyluridine
    CAS: 55486-09-4
    纯度: >98.00%

    A modified nucleoside

  • GC52303 structure
    GC52303Ethyl Mycophenolate
    CAS: 32483-51-5
    纯度: >98.00%

    A potential impurity found in commercial preparations of mycophenolate mofetil

  • GC52351 structure
    GC52351Citrullinated α-Enolase (R8 + R14) (1-19)-biotin Peptide

    A biotinylated and citrullinated α-enolase peptide

  • GC52391 structure
    GC52391306-O12B-3
    CAS: 2982842-90-8
    纯度: >95.00%

    An ionizable cationic lipidoid

  • GC60023 structure
    GC600233'-Deoxyuridine-5'-triphosphate
    CAS: 69199-40-2
    纯度: >98.00%

    3'-Deoxyuridine-5'-triphosphate(3'-dUTP)是一种核苷酸类似物，可抑制DNA依赖性RNA聚合酶I和II(RNApolymerasesI和II)，强烈并竞争性地抑制UTP掺入到RNA中，Ki值为2.0μM。

  • GC60031 structure
    GC600316-Azathymine
    CAS: 932-53-6
    纯度: >98.00%

    6-Azathymine，胸腺嘧啶的6-氮类似物，是一种有效的D-3-氨基异丁酸酯-丙酮酸氨基转移酶(D-3-aminoisobutyrate-pyruvateaminotransferase)抑制剂。6-Azathymine可抑制DNA的生物合成，并具有抗菌和抗病毒活性。

  • GC60062 structure
    GC60062AV-153 free base
    CAS: 19350-66-4
    纯度: >98.50%

    AV-153 free base 是一种 1,4-dihydropyridine (1,4-DHP) 衍生物，具有抗突变作用。在体外人细胞内，AV-153 free base 可以插入到 DNA 的单链断裂处，减少 DNA 损伤并刺激 DNA 修复。AV-153 free base 可以与胸腺嘧啶和胞嘧啶相互作用，对聚 (ADP) 核糖基化有影响。AV-153 free base 具有抗癌活性。

  • GC60089 structure
    GC60089Bleomycin hydrochloride
    CAS: 67763-87-5
    纯度: >98.00%

    Bleomycinhydrochloride是一种DNA损伤剂，抑制DNA合成。Bleomycinhydrochloride是一种抗肿瘤抗生素(antibiotic)。

  • GC60234 structure
    GC60234Maleic hydrazide
    CAS: 123-33-1
    纯度: >99.50%

    Maleichydrazide广泛用作植物生长调节剂和除草剂。Maleichydrazide是核酸和蛋白质合成的抑制剂。

  • GC60268 structure
    GC60268Neoxanthin
    CAS: 14660-91-4

    Neoxanthin是深绿色多叶蔬菜中的一种主要的叶黄素类胡萝卜素，是一种植物激素脱落酸的前体。Neoxanthin是一种有效的抗氧化剂和集光颜料，诱导细胞凋亡(apoptosis)并具有抗癌作用。

  • GC60310 structure
    GC60310Psammaplin A
    CAS: 110659-91-1
    纯度: >96.00%

    PsammaplinA是一种海洋代谢产物，是HDAC和DNA甲基转移酶(DNAmethyltransferases)的有效抑制剂。PsammaplinA是一种高效的选择性HDAC1抑制剂，IC50为0.9nM，对HDAC1的选择性是DAC6的360倍，对HDAC7和HDAC8的效力低1000倍以上。PsammaplinA对革兰氏阳性细菌(Gram-positivebacteria)具有抗菌作用，并抑制DNA合成和DNA促旋酶(DNAgyrase)活性。抗肿瘤活性。

  • GC60459 structure
    GC604592,4-D (2,4-Dichlorophenoxyacetic acid)
    CAS: 94-75-7

    A plant growth regulator

  • GC60616 structure
    GC60616AZT triphosphate
    CAS: 92586-35-1

    AZTtriphosphate(3'-Azido-3'-deoxythymidine-5'-triphosphate)是一种Zidovudine(AZT)的活性三磷酸酯代谢产物。AZTtriphosphate具有抗逆转录病毒的活性，并抑制HIV复制。AZTtriphosphate还可抑制HBV的DNA聚合酶。AZTtriphosphate可激活线粒体介导的凋亡(apoptosis)途径。

  • GC60617 structure
    GC60617AZT triphosphate TEA

    AZTtriphosphateTFA(3'-Azido-3'-deoxythymidine-5'-triphosphateTFA)是一种Zidovudine(AZT)的活性三磷酸酯代谢产物。AZTtriphosphateTFA具有抗逆转录病毒的活性，并抑制HIV复制。AZTtriphosphateTFA还可抑制HBV的DNA聚合酶。AZTtriphosphateTFA可激活线粒体介导的凋亡(apoptosis)途径。

  • GC60763 structure
    GC60763DHODH-IN-11
    CAS: 1263303-95-2
    纯度: >99.50%

    A derivative of leflunomide

  • GC61047 structure
    GC61047Methotrexate disodium
    CAS: 7413-34-5
    纯度: >98.00%

    Methotrexate sodium, an inhibitor of tetrahydrofolate dehydrogenase, is an antineoplastic antimetabolite with immunosuppressant properties.

  • GC61246 structure
    GC61246Rifalazil
    CAS: 129791-92-0
    纯度: >98.00%

    Rifalazil(ABI-1648;KRM-1648)是利福霉素衍生物，可抑制细菌依赖DNA的RNA聚合酶(RNApolymerase)并阻断RNA聚合酶中的β亚基从而杀死细菌感染的细胞。Rifalazil(ABI-1648;KRM-1648)是一种抗生素(antibiotic)，对分枝杆菌，革兰氏阳性细菌，幽门螺杆菌，肺炎衣原体和沙眼衣原体有抑制作用，其MIC值在0.00025至0.0025μg/ml之间。Rifalazil(ABI-1648;KRM-1648)有潜力用于衣原体感染，梭菌相关性腹泻菌感染 (CDAD)和结核病(TB)研究的相关研究。

  • GC61276 structure
    GC61276Silver sulfadiazine
    CAS: 22199-08-2
    纯度: >98.00%

    Silver sulfadiazine is a bactericidal for many gram- negative and gram-positive bacteria as well as being effective against yeast.

  • GC61348 structure
    GC61348Triazavirin
    CAS: 928659-17-0
    纯度: >99.00%

    An antiviral agent