Brassinazole (Brz) is a specific inhibitor of C-22 hydroxylase (CYP90B1) in brassinosteroid (BR) biosynthesis, and the application use of brassinazole has emerged as an effective way of complementing BR-deficient mutants to elucidate the functions of BRs[1]. Brassinazole has regulatory roles in G-fiber cell wall development and secondary xylem cell wall carbohydrate biosynthesis during tension wood formation in hybrid poplar[2]. In addition, brassinazole treatment (5µM) can delay persimmon fruit ripening and suppress respiration rate[3].
Brassinazole treatment (5, 1, and 0.5μM) markedly caused dwarfism of Arabidopsis seedlings in a concentration-dependent manner. Moreover, brassinazole treatment (1μM) showed a BR-deficient mutant-like phenotype, which is rescued by the application of brassinolide (BL) (10nM). Dark-grown 10-d-old Arabidopsis thaliana seedlings were treated with different concentrations of brassinozoles from 0 to 10μM . As the concentration of brassinozole increased, hypocotyl length decreased. The change in length occurred between 0.1 and 0.5μM[4].
[1]. Oh K, Matsumoto T, Yamagami A, Ogawa A, Yamada K, Suzuki R, et al. YCZ-18 is a new brassinosteroid biosynthesis inhibitor. PLoS One. 2015;10(3):e0120812. Epub 20150320. doi: 10.1371/journal.pone.0120812. PubMed PMID: 25793645.
[2]. Gao J, Yu M, Zhu S, Zhou L, Liu S. Effects of exogenous 24-epibrassinolide and brassinazole on negative gravitropism and tension wood formation in hybrid poplar (Populus deltoids X Populus nigra). Planta. 2019;249(5):1449-63. Epub 20190128. doi: 10.1007/s00425-018-03074-2. PubMed PMID: 30689054.
[3]. He Y, Li J, Ban Q, Han S, Rao J. Role of Brassinosteroids in Persimmon ( Diospyros kaki L.) Fruit Ripening. J Agric Food Chem. 2018;66(11):2637-44. Epub 20180312. doi: 10.1021/acs.jafc.7b06117. PubMed PMID: 29509414.
[4]. Asami T, Min YK, Nagata N, Yamagishi K, Takatsuto S, Fujioka S, et al. Characterization of brassinazole, a triazole-type brassinosteroid biosynthesis inhibitor. Plant Physiol. 2000;123(1):93-100. doi: 10.1104/pp.123.1.93. PubMed PMID.
芸苔素唑 (Brz) 是芸苔素类固醇 (BR) 生物合成中C-22羟化酶（CYP90B1）的特异性抑制剂，并且芸苔素唑的应用已成为补充BR缺陷突变体以阐明BRs功能的有效途径[1]。芸苔素唑在杂交杨树张力木材形成过程中的G纤维细胞壁发育和次生木质部细胞壁碳水化合物生物合成中具有调节作用[2]。另外，芸苔素唑处理 (5µM) 会延迟柿子果实成熟并抑制呼吸速率[3]。
芸苔素唑处理 (5, 1, 0.5μM) 会显著引起拟南芥幼苗矮化，且呈浓度依赖性。此外，芸苔素唑处理 (1μM) 显示出BR缺陷型突变体样表型，通过应用油菜素内酯 (BL) (10nM)可以挽救这种表型。用0至10μM不同浓度的芸苔素唑处理黑暗生长的10天龄拟南芥幼苗，随着芸苔素唑浓度的增加，下胚轴长度减少。长度变化发生在浓度 0.1至 0.5μM之间[4]。