Pinostrobin is a natural flavonoid with various biological activities, including antioxidant, anti-inflammatory, antiviral, and anticancer effects[1, 2]. Pinostrobin can inhibit nuclear factor-kappa B signaling and the production of inflammatory cytokines and chemokines in human macrophages[3].
In vitro, treatment of human cervical cancer HeLa cells with Pinostrobin (10-200µM) reduced cell viability and GSH and NO2- levels in a dose-dependent manner, induced apoptosis, significantly decreased mitochondrial membrane potential, and increased intracellular ROS production[4]. Treatment of 3T3-L1 and MCF-7 cells with Pinostrobin (25µM) significantly reduced adipogenesis in 3T3-L1 cells and inhibited migration and colony formation in MCF-7 cells[5]. Treatment of human acute myeloid leukemia (AML) cells with Pinostrobin (0-80µM) significantly reduced cell survival, with significant cytotoxic effects observed in MV4-11>MOLM-13>HL-60>U-937>THP-1 cells, affecting cell cycle progression and promoting apoptosis[6].
In vivo, treatment of zebrafish larvae with Pinostrobin (0-25µM) significantly reduced α-MSH-induced melanogenesis in a concentration-dependent manner without causing cardiotoxicity[7].
References:
[1] Gu C, Fu L, Yuan X, et al. Promoting effect of pinostrobin on the proliferation, differentiation, and mineralization of murine pre-osteoblastic MC3T3-E1 cells[J]. Molecules, 2017, 22(10): 1735.
[2] Chutiwitoonchai N, Akkarawongsapat R, Chantawarin S, et al. Antiviral effect of pinostrobin, a bioactive constituent of Boesenbergia rotunda, against porcine epidemic diarrhea virus[J]. Antiviral Research, 2025, 234: 106073.
[3] Budluang P, Potikanond S, Wikan N, et al. Pinostrobin Inhibits Nuclear Factor-Kappa B Signaling and Production of Inflammatory Cytokines and Chemokines in Human Macrophages[J]. Nutrients, 2025, 17(22): 3589.
[4] Jaudan A, Sharma S, Malek S N A, et al. Induction of apoptosis by pinostrobin in human cervical cancer cells: Possible mechanism of action[J]. PLoS One, 2018, 13(2): e0191523.
[5] Benchamana A, Tohkayomatee R, Soodvilai S, et al. Pinostrobin exerts inhibitory effects on adipogenesis and adipocyte-induced MCF-7 breast cancer cell proliferation and migration[J]. Trends in Sciences, 2024, 21(9): 8125-8125.
[6] Chen P Y, Lin C Y, Wu C L, et al. Pinostrobin modulates FOXO3 expression, nuclear localization, and exerts antileukemic effects in AML cells and zebrafish xenografts[J]. Chemico-Biological Interactions, 2023, 385: 110729.
[7] Athapaththu A M G K, Sanjaya S S, Lee K T, et al. Pinostrobin suppresses the α-melanocyte-stimulating hormone-induced melanogenic signaling pathway[J]. International Journal of Molecular Sciences, 2023, 24(1): 821.
Pinostrobin是一种天然类黄酮，具有抗氧化、抗炎、抗病毒和抗癌等多种生物活性[1, 2]。Pinostrobin能够抑制核因子-Kappa B信号传导及人类巨噬细胞中炎症细胞因子和化学因子的产生[3]。
在体外，Pinostrobin（10-200µM）处理人宫颈癌细胞HeLa细胞，以剂量依赖性方式降低了细胞活力以及GSH和NO2-水平，诱导了细胞凋亡，显著降低了线粒体膜电位，增加了细胞内ROS的生成[4]。Pinostrobin（25µM）处理3T3-L1细胞和MCF-7细胞，显著降低了3T3-L1细胞的脂肪生成，抑制了MCF-7细胞的迁移和集落形成[5]。Pinostrobin（0-80µM）处理人类急性骨髓性白血病（AML）细胞，显著降低了细胞的存活率，在 MV4-11>MOLM-13>HL-60>U-937>THP-1细胞中观察到明显的细胞毒性效应，影响了细胞周期进程，促进了细胞凋亡[6]。
在体内，Pinostrobin（0-25µM）处理斑马鱼幼体，以浓度依赖性方式显著降低了α-MSH诱导的斑马鱼幼体黑色素沉着，且不引起心脏毒性[7]。