Soyasaponin BB is an oleanane-type triterpenoid saponin derived from soybeans (a leguminous plant). As an orally active molecule, Soyasaponin BB exhibits a dual mechanism of action by inducing the expression of heme oxygenase-1 (HO-1) while simultaneously inhibiting the aldose reductase AKR1B1[1-2]. Soyasaponin BB helps reduce inflammatory responses and promotes cartilage tissue regeneration[3]. Soyasaponin BB can also alleviate LPS-induced cognitive dysfunction and intestinal inflammation[4].
In vitro, when C2C12 cells were co-cultured with Soyasaponin BB (10–30μg/mL) for 3 days under the induction of bone morphogenetic protein-2 (BMP-2; 100ng/mL), Soyasaponin BB promoted their differentiation into osteoblasts and significantly enhanced the activity and expression of alkaline phosphatase (ALP). Soyasaponin BB activates the AKT/MAPK signaling pathway, thereby promoting the transcription of downstream osteogenic markers such as osteocalcin (OCL) and type I collagen (Col1a)[5]. Pretreatment of BRL 3A rat hepatocytes with Soyasaponin BB (25–100μg/mL) for 24 hours, followed by stimulation with ethanol (250mmol/L) for another 24 hours, significantly alleviated ethanol-induced oxidative stress and hepatocyte damage[6].
In vivo, intragastric administration of Soyasaponin BB (10mg/kg; for 4 consecutive days) to male C57BL mice undergoing spontaneous withdrawal after chronic intermittent escalating-dose morphine administration significantly reduced withdrawal-induced anxiety-like behaviors[7]. Dietary supplementation with Soyasaponin BB (0.2g/100g feed) for 90 days in 60-day-old male pcy mice markedly delayed the progression of polycystic kidney disease[8].
References:
[1] Zhang Y, Chen F, Chen J, et al. Soyasaponin Bb inhibits the recruitment of toll-like receptor 4 (TLR4) into lipid rafts and its signaling pathway by suppressing the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-dependent generation of reactive oxygen species. Mol Nutr Food Res. 2016 Jul;60(7):1532-43.
[2] Lin X, Zhang C, Huang B. Hepatoprotective action mechanism and quantification of soyasaponin Bb in Abri Herba by HPLC and network pharmacology. J Ethnopharmacol. 2025 Jan 30;337(Pt 2):118850.
[3] Jiang Y, Li T, Liu B, et al. Soyasaponin Bb/Gelatin-Methacryloyl Hydrogel for Cartilage Inflammation Inhibition. ACS Omega. 2024 Dec 4;9(50):49597-49608.
[4] Yun SW, Lee DY, Park HS, et al. Heat-Processed Soybean Germ Extract and Lactobacillus gasseri NK109 Supplementation Reduce LPS-Induced Cognitive Impairment and Colitis in Mice. Nutrients. 2024 Aug 16;16(16):2736. doi: 10.3390/nu16162736.
[5] Kim SH, Yuk HJ, Ryu HW, et al. Biofunctional soyasaponin Bb in peanut (Arachis hypogaea L.) sprouts enhances bone morphogenetic protein-2-dependent osteogenic differentiation via activation of runt-related transcription factor 2 in C2C12 cells. Phytother Res. 2019 May;33(5):1490-1500.
[6] Lijie Z, Ranran F, Xiuying L, et al. Soyasaponin Bb Protects Rat Hepatocytes from Alcohol-Induced Oxidative Stress by Inducing Heme Oxygenase-1. Pharmacogn Mag. 2016 Oct-Dec;12(48):302-306.
[7] Wu J, Zhu C, Shu Y, et al. Soyasaponin Bb Attenuates Morphine Withdrawal-Induced Anxiety-Like Behaviors in Male Mice by Rescuing GSK-3β/Nrf2 Signaling. Psychiatry Investig. 2025 Jul;22(7):821-831.
[8] Philbrick DJ, Bureau DP, Collins FW, et al. Evidence that soyasaponin Bb retards disease progression in a murine model of polycystic kidney disease. Kidney Int. 2003 Apr;63(4):1230-9.
Soyasaponin BB是一种从豆科植物大豆中提取的三萜类齐墩果酸型皂，是一种口服有效的活性分子，兼具诱导血红素加氧酶-1(HO-1)表达与抑制醛糖还原酶AKR1B1的双重作用[1-2]。Soyasaponin BB有利于降低炎症反应，并促进软骨组织再生[3]。Soyasaponin BB可减轻LPS诱导的认知功能障碍和肠道炎症[4]。
在体外，Soyasaponin BB（10–30μg/mL）与C2C12细胞共培养3天，同时在骨形态发生蛋白-2（BMP-2；100ng/mL）诱导下促进其向成骨细胞分化，显著增强碱性磷酸酶（ALP）的活性和表达。Soyasaponin BB可激活AKT/MAPK信号通路，进而促进下游成骨标志分子如骨钙素（OCL）和Ⅰ型胶原（Col1a）的转录水平[5]。Soyasaponin BB（25–100μg/mL）预处理BRL 3A大鼠肝细胞24小时，随后以乙醇（250mmol/L）刺激24小时，Soyasaponin BB显著减轻了乙醇诱导的氧化应激和肝细胞损伤 [6]。
在体内，Soyasaponin BB（10mg/kg；连续4天）通过胃内给药处理经历慢性间歇性递增剂量吗啡给药后自发戒断的雄性C57BL小鼠，Soyasaponin BB显著减轻了戒断诱导的焦虑样行为[7]。Soyasaponin BB（0.2g/100g饲料）通过膳食补充方式处理60日龄雄性pcy小鼠90天，显著延缓了多囊肾病的疾病进展[8]。