2'-C-Methylcytidine (2CMC) is a ribonucleoside with broad-spectrum antiviral activity.1 It reduces the number of viral plaques in BHK-21 cells infected with dengue type 2, reovirus type 1, West Nile, and yellow fever RNA viruses with EC50 values of 95, 26, 80, and 75 μM, respectively. 2CMC inhibits hepatitis C virus (HCV) replication (EC50 = 2.2 μM in a replicon assay) and protects MDBK cells from infection with bovine virus diarrhea virus (BVDV; EC50 = 2.2 μM) and human coronavirus (HCoV; EC50 = 90 μM). It also reduces infectious virus yield in BHK-21 cells infected with foot-and-mouth disease virus (FMDV; EC50 = 6.4 μM) and swine vesicular disease virus (SVDV; EC50 = 45.2 μM).2 In vivo, 2CMC reduces viral shedding to undetectable levels in a mouse model of persistent norovirus infection.3
1.Benzaria, S., Bardiot, D., Bouisset, T., et al.2′-C-Methyl branched pyrimidine ribonucleoside analogues: Potent inhibitors of RNA virus replicationAntivir. Chem. Chemother.18(4)225-242(2007) 2.Goris, N., De Palma, A., Toussaint, J.F., et al.2'-C-methylcytidine as a potent and selective inhibitor of the replication of foot-and-mouth disease virusAntiviral Res.73(3)161-168(2007) 3.Rocha-Pereira, J., Van Dycke, J., and Neyts, J.Treatment with a nucleoside polymerase inhibitor reduces shedding of murine norovirus in stool to undetectable levels without emergence of drug-resistant variantsAntimicrob. Agents Chemother.60(3)1907-1911(2015)