n-Dodecyl β-D-maltoside, as a non-ionic detergent, has mild and non-denaturing properties used for during protein-anesthetic studies.[1]
In vitro, n-Dodecyl β-D-maltoside specifically interacts with HSA with Kd of 40 μM with a lower affinity than SDS with Kd of 2 μM. [1] In vitro efficacy test it shown that the magnitude of interactions between n-dodecyl-beta-D-maltoside and other surfactants followed the order anionic/nonionic > cationic/nonionic > nonionic/nonionic mixtures. In addition, electrolyte reduced synergism between n-dodecyl-beta-D-maltoside and ionic surfactant due to charge neutralization.[2] In vitro, after exposure to 2 mM n-Dodecyl β-D-maltoside, enterocytes were also generally well preserved, n-Dodecyl β-D-maltoside caused a obvious shortening of the microvilli of villus enterocytes compared with NaC.[3]
References:
[1]Xu L, et al. n-Dodecyl β-D-maltoside specifically competes with general anesthetics for anesthetic binding sites. J Biomol Struct Dyn. 2014;32(11):1833-40.
[2]Zhang R, et al. Study of mixtures of n-dodecyl-beta-D-maltoside with anionic, cationic, and nonionic surfactant in aqueous solutions using surface tension and fluorescence techniques. J Colloid Interface Sci. 2004 Oct 15;278(2):453-60.?
[3]Danielsen EM, et al. Probing the Action of Permeation Enhancers Sodium Cholate and N-dodecyl-β-D-maltoside in a Porcine Jejunal Mucosal Explant System. Pharmaceutics. 2018 Oct 2;10(4):172.?
n-Dodecyl β-D-maltoside 作为一种非离子洗涤剂,具有温和和非变性的特性,可用于蛋白质麻醉研究。[1]
在体外,正十二烷基 β-D-麦芽糖苷与 HSA 特异性相互作用,Kd 为 40 μM,亲和力低于 SDS,Kd 为 2 μM。 [1] 体外药效试验表明正十二烷基-β-D-麦芽糖苷与其他表面活性剂相互作用的大小顺序为阴离子/非离子>阳离子/非离子 >非离子/非离子混合物。此外,由于电荷中和,电解质降低了正十二烷基-β-D-麦芽糖苷和离子表面活性剂之间的协同作用。[2] 在体外,暴露于 2 mM 正十二烷基 β-D-麦芽糖苷后,肠细胞也普遍保存完好,与NaC相比,正十二烷基β-D-麦芽糖苷导致绒毛肠细胞的微绒毛明显缩短。[3]