MMP-2 Human Recombinant produced in HEK293 cells is a proform of the Human MMP-2 (Ala30-Cys660) and fused with a ployhistide tag at the C-terminus, having an Mw of 71kDa.
MMP-2 is purified by proprietary chromatographic techniques.
MMP-2 Human, HEK
目录号: GP25563纯度: >98%
Matrix Metalloproteinase-2 Human Recombinant, HEK
| 规格 | 价格 | 库存 | 数量 | 操作 |
|---|---|---|---|---|
| 2μg | ¥700.00 | 现货 | 1 | |
| 10μg | ¥1,820.00 | 现货 | 1 | |
| 1mg | ¥65,508.33 | 现货 | 1 |
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Nature641, 529–536 (2025)
Nature628, 630–638 (2024)
Nature632, 686–694 (2024)
Nature618, 1017–1023 (2023)
Nature610, 366–372 (2022)
Cell187(9):2288-2304 (2024)
Cell183(7):1867-1883 (2020)
Science388(6745) (2025)
Science387(6739) (2025)
Science387(6734) (2025)
Cell Research35, 97–116 (2025)
Cell Research34, 683–706 (2024)
Cell Research33, 273–287 (2023)
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Cell Research33, 904–922 (2023)
Cell Research31, 1291–1307 (2021)
产品描述 Description
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Purity:>98%
Product Data
Purity
Purity Greater than 95% as determined by SDS-PAGE.
Source
HEK293 cells.
Physical Appearance
The MMP-2 is supplied as a sterile Filtered colorless solution.
Shipping Condition
Shipped with Ice Packs
Synonyms
72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, MMP2, CLG4A, CLG4, MONA, MMP-II.
Stability
Store MMP-2 at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Biological Activity
The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001)., The specific activity is > 1,000 pmoles/min/µg.
Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).
Activation Protocol:
1. Dilute MMP2 to 100µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.
2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.
3. Incubate at 37°C for 1 hour.
Formulation
The MMP-2 is supplied as a 0.2µm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij 35, pH 7.4.
