MMP-2 Human, HEK

目录号: GP25563纯度: >98%

Matrix Metalloproteinase-2 Human Recombinant, HEK


MMP-2 Human, HEK
规格价格库存数量操作
2μg¥700.00现货
1
10μg¥1,820.00现货
1
1mg¥65,508.33现货
1

文献被引

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    Nature
    641, 529–536 (2025)
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    628, 630–638 (2024)
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    Nature
    632, 686–694 (2024)
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    Nature
    618, 1017–1023 (2023)
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    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
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    Science
    387(6739) (2025)
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    Science
    387(6734) (2025)
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    Cell Research
    35, 97–116 (2025)
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    34, 683–706 (2024)
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    33, 273–287 (2023)
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    33, 546–561 (2023)
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    33, 904–922 (2023)
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    31, 1291–1307 (2021)

产品描述 Description

MMP-2 Human Recombinant produced in HEK293 cells is a proform of the Human MMP-2 (Ala30-Cys660) and fused with a ployhistide tag at the C-terminus, having an Mw of 71kDa.
MMP-2 is purified by proprietary chromatographic techniques.

产品文档 Product Documents

Product Data

Purity
Purity Greater than 95% as determined by SDS-PAGE.
Source
HEK293 cells.
Physical Appearance
The MMP-2 is supplied as a sterile Filtered colorless solution.
Shipping Condition
Shipped with Ice Packs
Synonyms
72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, MMP2, CLG4A, CLG4, MONA, MMP-II.
Stability
Store MMP-2 at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Biological Activity
The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001)., The specific activity is > 1,000 pmoles/min/µg. Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA). Activation Protocol: 1. Dilute MMP2 to 100µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5. 2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO. 3. Incubate at 37°C for 1 hour.
Formulation
The MMP-2 is supplied as a 0.2µm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij 35, pH 7.4.