luteolin-6-C-glucoside

Cell experiment:

PANC-1 and PATU-8988 cells are plated onto 96-well plates. Each well contain ~5,000 cells and 200 μL of the medium with 10% FBS. When the cells of each well reach 70% confluency, the medium is changed, and FBS-free medium with different concentrations of Isoorientin is added. After 24 hours, the cells are washed with PBS once, the medium containing Isoorientin is discarded, and 100 μL of FBS-free medium with 10 μL of the Cell Counting Kit 8 (CCK8) reagent are added. The cells are incubated for another 1-2 hours at 37°C, and the absorbance of each well is detected using an ELISA reader at 490 nm. Cell viability is expressed as the fold change of absorbance[2].

Animal experiment:

Mice[3]In the case of paw edema model the Isoorientin or Celecoxib is given intraperitoneally and Carrageenan is injected into the paw directly one hour later. In air pouch model all the treatments are given along with Carrageenan directly into the pouch cavity. Isoorientin is injected three hours earlier than injection of Carrageenan into the pouch cavity. Isoorientin and Celecoxib are administered into the mice air pouch. The stock solutions of Isoorientin (100 mg/mL) and Celecoxib (100 mg/mL) are prepared in DMSO and further dilutions are made at the time of treatments. Animals are divided, into 5 different groups as follows: control (DMSO treated); Carrageenan (0.5 mL of 1.5% (w/v) Carrageenan in saline) treated; Carrageenan+Celecoxib (20 mg/kg body weight) treated; Carrageenan+Isoorientin (10 mg/Kg body weight) treated; Carrageenan+Isoorientin (20 mg/Kg body weight) treated.

References:

[1]. Sumalatha M, et al. Isoorientin, a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa. Nat Prod Commun. 2015 Oct;10(10):1703-4.
[2]. Ye T, et al. Isoorientin induces apoptosis, decreases invasiveness, and downregulates VEGF secretion by activating AMPK signaling in pancreatic cancer cells. Onco Targets Ther. 2016 Dec 12;9:7481-7492.
[3]. Anilkumar K, et al. Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa. Oxid Med Cell Longev. 2017;2017:5498054.