H2S (Hydrogen sulfide) Donor 5a, as a stable, cysteine activated hydrogen sulfide donor, can react with reducing agents containing sulfhydryl groups such as cysteine and reduced glutathione to release H2S. H2S Donor 5a is very stable in aqueous solution, however, it will degrade and release H2S in biological samples containing thiols such as blood, tissues or cells.[1]
H2S exerts cytoprotective and anticancer effects, promotes wound healing, inhibits platelet aggregation and protects against myocardial ischemia, etc.[2]
In vitro experiment it shown that 0.5 mg/mL and 2 mg/mL of the most promising material (5A@Chi) was loaded with H2S and added to the HeLa cells. Both concentrations, which correspond to a H2S release of 0.49 μM and 2.20 μM, respectively, indicated no toxicity after 48 h to the cells.[3]
References:
[1]Zhao Y, et al. Cysteine-activated hydrogen sulfide (H2S) donors. J Am Chem Soc. 2011 Jan 12;133(1):15-7.
[2]Zheng Y., et al. Toward hydrogen sulfide based therapeutics: Critical drug delivery and developability issues. Med. Res. Rev. 2018;38:57–100.
[3]Batista M, et al. Chitosan Biocomposites for the Adsorption and Release of H2S. Materials (Basel). 2021 Nov 7;14(21):6701.
H2S（硫化氢）供体 5a 作为稳定的半胱氨酸活化硫化氢供体,可以与半胱氨酸和还原型谷胱甘肽等含有巯基的还原剂反应,释放出 H2S。 H2S Donor 5a 在水溶液中非常稳定,但在血液、组织或细胞等含有硫醇的生物样品中会降解并释放 H2S。[1]
H2S具有细胞保护和抗癌作用,促进伤口愈合,抑制血小板聚集,防止心肌缺血等。[2]
体外实验表明,0.5 mg/mL 和 2 mg/mL 最有前途的材料 (5A@Chi) 负载 H2S 并添加到 HeLa 细胞中。这两个浓度分别对应于 0.49 μM 和 2.20 μM 的 H2S 释放,表明 48 小时后对细胞没有毒性。[3]