Guanylic acid (5'-GMP) is an important nucleotide compound and a component of RNA[1-2]. Guanylic acid is involved in various metabolic pathways and exhibits certain biological activities, such as potential roles in regulating nutrient metabolism and enhancing immunity[3-4].
In vitro, treating L5178Y cells with Guanylic acid (40-200μM) and Bredinin (50-100μM) for 16–40 hours reversed the inhibitory effects of Bredinin on nucleic acid synthesis and chromosomal aberrations in L5178Y cells. Guanylic acid also caused an increase in cell volume and inhibited cell proliferation in L5178Y cells[5].
In vivo, treating adult male Wistar rats with Guanylic acid (10–150mg/kg; intraperitoneal injection) 1 hour prior to testing significantly increased anxiolytic-like behavior. Guanylic acid did not alter cerebrospinal fluid purine concentration or locomotor activity in the rat[6].
References:
[1] Tansutaphanit S, Haga Y, Kabeya N, et al. Impact of purine nucleotide on fatty acid metabolism and expression of lipid metabolism-related gene in the liver cell of rainbow trout Oncorhynchus mykiss. Comp Biochem Physiol B Biochem Mol Biol. 2023 Jun-Jul;266:110845.
[2] FAIRLEY JL Jr, LORING HS. Growth-promoting activities of guanine, guanosine, guanylic acid, and xanthine for a purine-deficient strain of Neurospora. J Biol Chem. 1949 Jan;177(1):451-9.
[3] Ni X, Han Y, Yu J, et al. Structural basis of the C-terminal domain of SARS-CoV-2 N protein in complex with GMP reveals critical residues for RNA interaction. Bioorg Med Chem Lett. 2024 Dec 1;114:130014.
[4] Molz S, Dal-Cim T, Tasca CI. Guanosine-5'-monophosphate induces cell death in rat hippocampal slices via ionotropic glutamate receptors activation and glutamate uptake inhibition. Neurochem Int. 2009 Dec;55(7):703-9.
[5] Sakaguchi K, Tsujino M, Hayashi M, et al. Mode of action of bredinin with guanylic acid on L5178Y mouse leukemia cells. J Antibiot (Tokyo). 1976 Dec;29(12):1320-7.
[6] Almeida RF, Cereser VH Jr, Faraco RB, et al. Systemic administration of GMP induces anxiolytic-like behavior in rats. Pharmacol Biochem Behav. 2010 Sep;96(3):306-11.
Guanylic acid (5'-GMP)是一种重要的核苷酸类化合物，是RNA的组成成分[1-2]。Guanylic acid与多种代谢途径有关，并具有一定的生物活性，如研究表明其可能具有调节机体营养代谢、提高免疫力等潜力[3-4]。
在体外，Guanylic acid（40-200μM）与Bredinin（50-100μM）共同处理L5178Y细胞16–40小时，可逆转Bredinin对L5178Y细胞核酸合成的抑制及染色体畸变，Guanylic acid还引起L5178Y细胞体积增大并抑制细胞增殖[5]。
在体内，Guanylic acid（10–150mg/kg；腹腔注射）处理成年雄性Wistar大鼠1小时，Guanylic acid显著增加小鼠的的抗焦虑样行为，且Guanylic acid未改变小鼠脑脊液嘌呤浓度或运动活性[6]。