Soyasapogenol A是一种从Abrus cantoniensis根中分离得到的三萜类化合物，具有抗炎、抗癌、保肝和抗HIV-1等活性。
Cas No.:508-01-0
Sample solution is provided at 25 µL, 10mM.
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Soyasapogenol A is a triterpenoid compound isolated from the roots of Abrus cantoniensis that exhibits anti-inflammatory, anti-cancer, hepatoprotective, and anti-HSV-1 activities. Soyasapogenol A can be used in the research of tumors and immune inflammatory diseases[1][2][3].
In vitro, treatment with Soyasapogenol A at 10µM for 11 days caused a 68% reduction in MDA-MB-231 cell proliferation, whereas it stimulated a 2.4-fold increase in MCF-7 cell proliferation[4]. Treatment with Soyasapogenol A for 24h increased pS2 mRNA expression in MCF-7 cells by 3.4-fold at 0.1µM and by 5.5-fold at 1µM[4]. Treatment with Soyasapogenol A (0-0.5mg/mL) for 24-72h inhibited the proliferation of Hep-G2 cells, with an LC₅₀ of 0.052mg/mL, and induced cell cycle arrest and apoptosis[1].
In vivo, Soyasapogenol A was orally administered at a dose of 15mg/kg twice weekly for 6 weeks in SKOV-3 xenograft mice, demonstrating a potent and significant reduction in the growth of subcutaneous xenografts by more than 55% and lung metastases by more than 60%[5].
References:
[1] Zhang W, et al. Effect of soyasapogenol A and soyasapogenol B concentrated extracts on HEP-G2 cell proliferation and apoptosis. J Agric Food Chem. 2008 Apr 23;56(8):2603-8.
[2] Kuzuhara H, et al. Protective effects of soyasapogenol A on liver injury mediated by immune response in a concanavalin A-induced hepatitis model. Eur J Pharmacol. 2000 Mar 10;391(1-2):175-81.
[3] Guang C, et al. Biological functionality of soyasaponins and soyasapogenols. J Agric Food Chem. 2014 Aug 20;62(33):8247-55.
[4] Rowlands JC, Berhow MA, Badger TM. Estrogenic and antiproliferative properties of soy sapogenols in human breast cancer cells in vitro. Food Chem Toxicol. 2002;40(12):1767-1774.
[5]Omar A, Kalra RS, Putri J, Elwakeel A, Kaul SC, Wadhwa R. Soyasapogenol-A targets CARF and results in suppression of tumor growth and metastasis in p53 compromised cancer cells. Sci Rep. 2020;10(1):6323.
Soyasapogenol A是一种从Abrus cantoniensis根中分离得到的三萜类化合物，具有抗炎、抗癌、保肝和抗HIV-1等活性。Soyasapogenol A可用于肿瘤和免疫炎性疾病的研究[1][2][3]。
体外实验中，Soyasapogenol A在10µM浓度下处理11天，MDA-MB-231细胞增殖率降低68%，而MCF-7细胞增殖率增加2.4倍[4]。Soyasapogenol A处理24h后，MCF-7细胞中pS2 mRNA的表达在0.1µM时增加3.4倍，在1µM时增加5.5倍[4]。Soyasapogenol A（0-0.5mg/mL）处理24-72h抑制Hep-G2细胞的增殖，其LC50值为0.052mg/mL，并诱导细胞周期阻滞和凋亡[1]。
体内实验中，Soyasapogenol A以15mg/kg的剂量口服给予SKOV-3异种移植小鼠，每周两次，持续6周，显著且强效地抑制了肿瘤进展，使皮下移植瘤的生长减少超过55%，肺转移减少超过60%[5]。
| Cell experiment [1]: | |
Cell lines | MCF-7 and MDA-MB-231 |
Preparation Method | Cell suspensions were diluted into a 24-well plate. The following day, Soyasapogenol A dissolved in DMSO were added. The final DMSO concentration did not exceed 0.3% (v/v). The cells were incubated in the Soyasapogenol A for a further 11 days in a 5% CO2 atmosphere and 100% humidity. The media with chemicals was changed after 4 days and then every 2 days thereafter. |
Reaction Conditions | 0, 0.1, 1, 10µM; 11d |
Applications | There were no significant changes in proliferation of MCF-7 or MDA-MB-231 cells cultured in 0.1 or 1.0µM SA for 11 days. However, at 10µM , Soyasapogenol A caused 68% reduction in MDA-MB-231 cell proliferation, whereas it stimulated a 2.4-fold increase in MCF-7 cell proliferation. |
| Animal experiment [2]: | |
Animal models | Athymic balb/c nude female mice transplanted with SKOV3 cells |
Preparation Method | Animals were pre-fed with either vehicle (0.1% carboxymethyl cellulose) or Soyasapogenol A supplemented vehicle (15mg/kg) in 250µL suspension twice a week before xenografting of SKOV-3 cells by subcutaneous and intravenous injections. Post-1 week of injections, mice were feed with Soyasapogenol A for next 4 weeks, before sacrifice. |
Dosage form | 15mg/kg; 2/w, 6w; p.o. |
Applications | Soyasapogenol A fed mice demonstrated a potent and significant reduction (>55%) in growth of subcutaneous xenografts and lung metastases (>60%). |
References: | |
| Cas No. | 508-01-0 | SDF | |
| 别名 | 大豆甾醇A | ||
| Canonical SMILES | C[C@]12[C@]3(C([C@@]4([H])[C@](C)([C@@H]([C@H](O)C(C)(C)C4)O)CC3)=CC[C@]1([H])[C@@]5([C@@]([C@](C)([C@@H](O)CC5)CO)([H])CC2)C)C | ||
| 分子式 | C30H50O4 | 分子量 | 474.72 |
| 溶解度 | DMSO : 100 mg/mL (210.65 mM; Need ultrasonic) | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1065 mL | 10.5325 mL | 21.065 mL |
| 5 mM | 421.3 μL | 2.1065 mL | 4.213 mL |
| 10 mM | 210.7 μL | 1.0533 mL | 2.1065 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
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