Luciferase is an enzyme capable of catalyzing bioluminescent reactions. Luciferase catalyzes the oxidative decarboxylation of the substrate luciferin to convert chemical energy into light energy, and it can be used to reflect the expression level of a target gene by being coupled with that gene. Luciferase can be used in research related to gene expression regulation and in vivo imaging[1-4].
References:
[1] Schramm S, Al-Handawi MB, Karothu DP, et al. Mechanically Assisted Bioluminescence with Natural Luciferase. Angew Chem Int Ed Engl. 2020 Sep 14;59(38):16485-16489.
[2] Tu SC. Activity coupling and complex formation between bacterial luciferase and flavin reductases. Photochem Photobiol Sci. 2008 Feb;7(2):183-8.
[3] Dupont S. Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells. Methods Mol Biol. 2019;1893:121-135.
[4] Yang T, Yuan Y, Ma F. Luciferase Reporter Systems in Investigating Interferon Antiviral Innate Immunity. Methods Mol Biol. 2025;2854:127-141.
Luciferase是一种能够催化生物发光反应的酶。Luciferase可催化底物荧光素氧化脱羧将化学能转化为光能，同时通过偶联目的基因以反映其表达水平。Luciferase可用于基因表达调控和活体成像的相关研究[1-4]。