DAF-FM DA (Diaminofluorescein-FM diacetate) is a cell-permeable fluorescent probe used for the detection and bioimaging of nitric oxide (NO). The DAF-FM DA (Diaminofluorescein-FM diacetate) probe can quantify the level of nitric oxide (NO) in cells, with a maximum excitation/emission wavelength of 495/515nm[1-2].
DAF-FM DA (Diaminofluorescein-FM diacetate) can permeate the cell membrane of living cells and is deacetylated by intracellular esterases to become DAF-FM. DAF-FM can react with NO produced in cells to form highly fluorescent triazole fluorescein (DAF-FMT), which can be located and monitored by fluorescence or confocal microscopy, or detected by fluorescence assay and flow cytometry. The NO detection limit of DAF-FM DA (Diaminofluorescein-FM diacetate) is approximately 3nM[1-3]. DAF-FM DA (Diaminofluorescein-FM diacetate) can be used as a vital dye for zebrafish embryos to mark the early ossification centers of developing zebrafish[4]. DAF-FM DA (Diaminofluorescein-FM diacetate) can be used to detect the production of NO in fungi[5].
References:
[1] Kojima, H., Urano, Y., Kikuchi, K., et al.Fluorescent indicators for imaging nitric oxide productionAngew. Chem. Int. Ed. Engl.38(21)3209-3212(1999)
[2] Kojima, H., Nakatsubo, N., Kikuchi, J., et al.Detection and imaging of nitric oxide with novel fluorescent indicators: DiaminofluoresceinsAnal. Chem.70(13)2446-2453(1998)
[3] Kumari A, Bhatoee M, Singh P, et al. Detection of nitric oxide from chickpea using DAF fluorescence and chemiluminescence methods[J]. Current Protocols, 2022, 2(4): e420.
[4] Huysseune A, Larsen U G, Larionova D, et al. Bone formation in zebrafish: the significance of DAF-FM DA staining for nitric oxide detection[J]. Biomolecules, 2023, 13(12): 1780.
[5] Oiki S, Nasuno R, Urayama S, et al. Intracellular production of reactive oxygen species and a DAF-FM-related compound in Aspergillus fumigatus in response to antifungal agent exposure[J]. Scientific Reports, 2022, 12(1): 13516.
DAF-FM DA (Diaminofluorescein-FM diacetate)是一种细胞渗透性荧光探针，用于一氧化氮（NO）的检测和生物成像，DAF-FM DA (Diaminofluorescein-FM diacetate)探针可量化细胞中的一氧化氮（NO）水平，最大激发/发射波长为495/515nm[1-2]。
DAF-FM DA (Diaminofluorescein-FM diacetate)可渗透活细胞的细胞膜，在细胞内被细胞内酯酶脱乙酰化，成为DAF-FM，DAF-FM可与细胞内产生的NO发生反应，形成高荧光三唑荧光素（DAF-FMT），可以通过荧光或共聚焦显微镜进行定位和监测，也可以通过荧光测定法和流式细胞术进行检测。DAF-FM DA (Diaminofluorescein-FM diacetate)的NO检测限约为3nM[1-3]。DAF-FM DA (Diaminofluorescein-FM diacetate)可作为斑马鱼胚胎的活体染料，用于标记发育中的斑马鱼早期骨化中心[4]。DAF-FM DA (Diaminofluorescein-FM diacetate)可用于检测真菌中NO的产生[5]。