BODIPY 558/568 C12 is a fatty acid-conjugated fluorescent probe for lipid droplets[1] [2]. BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) is a small molecule dye characterized by strong absorption and emission in the visible and near-infrared regions, strong fluorescence, and supreme photostability[3]. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets. which can be used for labeling of live cells and fixed cells[4]. BODIPY 558/568 C12, as a BODIPY dye, displays excitation/emission maxima of 558/568nm, respectively, and has been used to monitor the localization and dynamics of lipid droplets in live cells[5-7].
References:
[1] Targett-Adams, P., Chambers, D., Gledhill, S., Hope, R. G., Coy, J. F., Girod, A., & McLauchlan, J. (2003). Live cell analysis and targeting of the lipid droplet-binding adipocyte differentiation-related protein. The Journal of biological chemistry, 278(18), 15998–16007.
[2] Rambold, A. S., Cohen, S., & Lippincott-Schwartz, J. (2015). Fatty acid trafficking in starved cells: regulation by lipid droplet lipolysis, autophagy, and mitochondrial fusion dynamics. Developmental cell, 32(6), 678–692.
[3] Das, S., Dey, S., Patra, S., Bera, A., Ghosh, T., Prasad, B., Sayala, K. D., Maji, K., Bedi, A., & Debnath, S. (2023). BODIPY-Based Molecules for Biomedical Applications. Biomolecules, 13(12), 1723.
[4] Yu, Y., Maguire, T. G., & Alwine, J. C. (2012). Human cytomegalovirus infection induces adipocyte-like lipogenesis through activation of sterol regulatory element binding protein 1. Journal of virology, 86(6), 2942–2949.
[5] Ioannou, M. S., Liu, Z., & Lippincott-Schwartz, J. (2019). A Neuron-Glia Co-culture System for Studying Intercellular Lipid Transport. Current protocols in cell biology, 84(1), e95.
[6] Qi, G., Mi, Y., Shi, X., Gu, H., Brinton, R. D., & Yin, F. (2021). ApoE4 Impairs Neuron-Astrocyte Coupling of Fatty Acid Metabolism. Cell reports, 34(1), 108572.
[7] Surendradoss, J., Chang, T. K., & Abbott, F. S. (2014). Evaluation of in situ generated valproyl 1-O-β-acyl glucuronide in valproic acid toxicity in sandwich-cultured rat hepatocytes. Drug metabolism and disposition: the biological fate of chemicals, 42(11), 1834–1842.
BODIPY 558/568 C12 是一种用于标记脂滴的脂肪酸偶联荧光探针[1][2]。BODIPY（4,4-二氟-4-硼-3a,4a-二氮杂-s-茚）是一种小分子染料，其特征是在可见光和近红外区域具有强大的吸收和发射能力、强荧光以及卓越的光稳定性[3]。BODIPY 脂滴染料能够很好地穿过细胞膜进入细胞，并在细胞内定位中性脂质，从而特异性地染色脂滴，可用于活细胞和固定细胞的标记[4]。作为一种BODIPY染料，BODIPY 558/568 C12 的激发/发射最大值分别为558/568nm，并且已被用于监测活细胞中脂滴的定位和动态变化[5-7]。