Monochlorobimane (Chlorobimane) is a non-fluorescent cell-permeant probe at 380nm wavelength excitation and 470nm wavelength emission[1]. Once inside the cell, Monochlorobimane reacts with reduced glutathione (GSH) to form fluorescent bimane–glutathione (B-SG) adducts, in a reaction catalyzed by glutathione S-transferase[2]. Monochlorobimane has been widely used for detecting GSH levels and screening bacterial populations that express glutathione transferase (GST)[3].
In vivo, intracranially inject 10μg/5μl of Monochlorobimane at a rate of 5μl/3min for 30 minutes, which significantly reduced the staining of cytochrome c oxidase (COX) and succinate dehydrogenase (SDH) in the hippocampus of rats induced by microsphere embolism (ME)[4].
References:
[1] Kamencic H, Lyon A, Paterson P G, et al. Monochlorobimane fluorometric method to measure tissue glutathione[J]. Analytical biochemistry, 2000, 286(1): 35-37.
[2] Machado M D, Soares E V. Assessment of cellular reduced glutathione content in Pseudokirchneriella subcapitata using monochlorobimane[J]. Journal of Applied Phycology, 2012, 24(6): 1509-1516.
[3] Eklund B I, Edalat M, Stenberg G, et al. Screening for recombinant glutathione transferases active with monochlorobimane[J]. Analytical biochemistry, 2002, 309(1): 102-108.
[4] Oshikawa S, Miyake-Takagi K, Takagi N, et al. Effects of monochlorobimane on cerebral ischemia-induced damage to mitochondria[J]. Biological and Pharmaceutical Bulletin, 2004, 27(2): 166-169.
Monochlorobimane (Chlorobimane)是一种非荧光细胞渗透性探针，激发波长为380nm，发射波长为470nm[1]。Monochlorobimane进入细胞后，在谷胱甘肽S-转移酶(GST)的催化下与还原型谷胱甘肽反应，形成具有荧光的硼烷-谷胱甘肽(B-SG)加合物[2]。Monochlorobimane已广泛应用于检测细胞内谷胱甘肽水平及筛选表达GST的细菌群体[3]。
在体内，以5μl/3分钟的速率向大鼠颅内注射10μg/5μl的Monochlorobimane持续30分钟，可显著减轻微球栓塞诱导的海马区细胞色素c氧化酶和琥珀酸脱氢酶染色强度[4]。