(±)8,9-DiHET-d11 contains 11 deuterium atoms at the 16, 16, 17, 17, 18, 18, 19, 19, 20, 20, and 20 positions. It is intended for use as an internal standard for the quantification of (±)8,9-DiHET by GC- or LC-mass spectrometry. Epoxide hydrolases convert the EETs into vicinal diols, with the concurrent loss of much of their biological activity.1 The 8(S),9(R)-EET isomer is metabolized by platelet COX to form 8(S),9(R),11(R)-THETA, a trihydroxy fatty acid which may act as a renal vasoconstrictor.2
1.Oliw, E.H., Guengerich, F.P., and Oates, J.A.Oxygenation of arachidonic acid by hepatic monooxygenases. Isolation and metabolism of four epoxide intermediatesJ. Biol. Chem.257(7)3771-3781(1982)
2.Zhang, J.Y., Prakash, C., Yamashita, K., et al.Regiospecific and enantioselective metabolism of 8,9-epoxyeicosatrienoic acid by cyclooxygenaseBiochem. Biophys. Res. Commun.183(1)138-143(1992)
