APF is a novel ROS indicator that selectively and dose-dependently fluoresces upon binding to certain reactive oxygen species in solution and in cells with a maximum excitation/emission wavelength of 490/515 nm. APF has higher specificity and stability than HDCFDA. APF is cell membrane permeable, non-fluorescent, and reacts with reactive oxygen species, such as hydroxyl radical, peroxynitroso anion, or hypochlorite anion, to produce strong green fluorescence, which can be detected by fluorescence microscopy or flow cytometry. After reacting with hydroxyl radicals, peroxynitrite anion or hypochlorite anion, APF produces strong green fluorescence, which can be detected by fluorescence microscope, high throughput imager, fluorescence zymography or flow cytometry. At the same time, it can also be coupled with a variety of molecules to achieve specific labeling detection, which is widely used in the fields of biomarkers, cell imaging and fluorescence microscopy[1].
References:
[1]. Cohn C A, Pedigo C E, Hylton S N, et al. Evaluating the use of 3'-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions[J]. Geochemical Transactions, 2009, 10: 1-9.
APF是一种新型ROS指示剂，可选择性、剂量依赖性地与溶液和细胞中某些活性氧结合后产生荧光，其最大激发/发射波长分别为490/515nm。APF比HDCFDA具有更高的特异性和稳定性。APF具有细胞膜渗透性，本身无荧光，与羟基自由基、过氧亚硝基阴离子或次氯酸阴离子等活性氧物质反应后产生强绿色荧光，可用荧光显微镜，高通量成像仪，荧光酶标板或流式细胞仪检测。同时，它还可以与多种分子偶联，实现特异性标记检测，广泛应用于生物标